Molecular characterization, balancing selection, and genomic organization of the tree shrew (Tupaia belangeri) MHC class I gene

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 分子免疫药理学

	Molecular characterization, balancing selection, and genomic organization of the tree shrew (Tupaia belangeri) MHC class I gene
	Zhang XH 1,2; Dai ZX 1; Zhang GH 1; Han JB 1; Zheng YT[*]1
刊名	GENE
	2013
卷号	522 期号:2 页码:147-155
关键词	Tupaia belangeri Tree shrew Allele Splice variant Haplotype
通讯作者	zhengyt@mail.kiz.ac.cn
合作状况	其它
英文摘要	The major histocompatibility complex (MHC) class I genes play a pivotal role in the adaptive immune response among vertebrates. Accordingly, in numerous mammals the genomic structure and molecular characterization of MHC class I genes have been thoroughly investigated. To date, however, little is known about these genes in tree shrews, despite the increasingly popularity of its usage as an animal model. To address this deficiency, we analyzed the structure and characteristic of the tree shrew MHC class I genes (Tube-MHC I) and performed a comparative gene analysis of the tree shrew and other mammal species. We found that the full-length cDNA sequence of the tree shrew MHC class I is 1074 bp in length. The deduced peptide is composed of 357 amino acids containing a leader peptide, an alpha 1 and alpha 2 domain, an alpha 3 domain, a transmembrane domain and a cytoplasmic domain. Among these peptides, the cysteines, CD8(+) interaction and N-glycosylation sites are all well conserved. Furthermore, the genomic sequence of the tree shrew MHC class I gene was identified to be 3180 bp in length, containing 8 exons and 7 introns. In 21 MHC class I sequences, we conducted an extensive study of nucleotide substitutions. The results indicated that in the peptide binding region (PBR) the rate of non-synonymous substitutions (dN) to synonymous substitutions (dS) was greater than 1, suggesting balancing selection at the PBR. These findings provide valuable contributions in furthering our understanding of the structure, molecular polymorphism, and function of the MHC class I genes in tree shrews, further improving their utility as an animal model in biomedical research
收录类别	SCI
资助信息	This work was supported by the 863 Program of China (2012AA021801), the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-J-23), the National Basic Research Program of China (2012CBA01305), and the National Natural Science Foundation of China (30872317; 30800113; U0832601).
语种	英语
WOS记录号	WOS:000320147000004
公开日期	2013-07-18
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7573]
专题	昆明动物研究所_分子免疫药理学昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Key laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.University of Chinese Academy of Sciences, Beijing 100049, China
推荐引用方式 GB/T 7714	Zhang XH,Dai ZX,Zhang GH,et al. Molecular characterization, balancing selection, and genomic organization of the tree shrew (Tupaia belangeri) MHC class I gene[J]. GENE,2013,522(2):147-155.
APA	Zhang XH,Dai ZX,Zhang GH,Han JB,&Zheng YT[*].(2013).Molecular characterization, balancing selection, and genomic organization of the tree shrew (Tupaia belangeri) MHC class I gene.GENE,522(2),147-155.
MLA	Zhang XH,et al."Molecular characterization, balancing selection, and genomic organization of the tree shrew (Tupaia belangeri) MHC class I gene".GENE 522.2(2013):147-155.