Circulating immunoglobulin A- and immunoglobulin G-secreting hybridoma cells in peripheral blood preferably migrate to female genital tracts. The role of sex hormones | |
Wang XL; Zhao XD; Ben KL[*]; Cao XM; Wang YQ; Zhou HM | |
刊名 | IMMUNOLOGY
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2002 | |
卷号 | 106期号:3页码:343-353 |
ISSN号 | 0019-2805 |
通讯作者 | kben@mail.kiz.ac.cn |
合作状况 | 其它 |
英文摘要 | Antigen-specific circulating immunoglobulin-secreting cells (ISC) migrate to various secondary and tertiary lymphoid tissues. To understand the migration of the cells into the genital tract and its regulation by sex hormones, spleen-derived SG2 hybridoma cells secreting immunoglobulin G2b (IgG2b) and Peyer's patch-derived PA4 hybridoma cells secreting polymer IgA were labelled with (3) H-TdR, and intravenously injected into syngeneic mice of both sexes. Using flow cytometry, surface molecular markers of plasma cells, CD38 and CD138, and adhesion molecules, CD49d, CD162, and CD11a were found to be positive in SG2 and PA4 cells, but CD62L, alpha4beta7 and CD44 were not expressed on these cells. The relative distribution indexes (RDIs) of the cells in genital tract and other tissues were measured. The means of RDIs of SG2 and PA4 cells in female genital tissues were 6.5 and 4.5 times as many as the means in male genital tissues, respectively. The treatment of ovariectomized mice with beta-oestradiol significantly increased the RDIs of PA4 cells in cervix and vagina, but decreased the RDIs of SG2 cells in vagina, horn of uterus, uterus and rectum (P <0.05). Progesterone treatment increased the RDIs of PA4 cells in vagina and rectum (P <0.05). The treatment with testosterone significantly increased the RDIs of SG2 and PA4 cells in epididymis and accessory sex glands (P <0.05). These results demonstrate that the female genital tract is the preferable site for the migration of circulating hybridoma cells to the male genital tract, and sex hormones play an important role in regulation of the migration of circulating ISC to genital tracts. |
收录类别 | SCI |
资助信息 | This work was supported by National Natural Science Foundation of China (39600016), Applied and Basic Research Fund of Yunnan (96C097Q), and Rockefeller Foundation (88045). |
原文出处 | 2002106343.pdf |
语种 | 英语 |
公开日期 | 2010-08-24 |
内容类型 | 期刊论文 |
源URL | [http://159.226.149.42:8088/handle/152453/5239] ![]() |
专题 | 昆明动物研究所_其他 昆明动物研究所_肿瘤干细胞生物学 |
作者单位 | Laboratory for Molecular and Cell Immunology, Kunming Institute of Zoology, The Chinese Academy of Sciences, China |
推荐引用方式 GB/T 7714 | Wang XL,Zhao XD,Ben KL[*],et al. Circulating immunoglobulin A- and immunoglobulin G-secreting hybridoma cells in peripheral blood preferably migrate to female genital tracts. The role of sex hormones[J]. IMMUNOLOGY,2002,106(3):343-353. |
APA | Wang XL,Zhao XD,Ben KL[*],Cao XM,Wang YQ,&Zhou HM.(2002).Circulating immunoglobulin A- and immunoglobulin G-secreting hybridoma cells in peripheral blood preferably migrate to female genital tracts. The role of sex hormones.IMMUNOLOGY,106(3),343-353. |
MLA | Wang XL,et al."Circulating immunoglobulin A- and immunoglobulin G-secreting hybridoma cells in peripheral blood preferably migrate to female genital tracts. The role of sex hormones".IMMUNOLOGY 106.3(2002):343-353. |
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