Phenotype and Function of Monocyte-Derived Dendritic Cells from Chinese Rhesus Macaques | |
Xia HJ1,2; Liu HL1,2; Zhang GH1; Zheng YT[*]1 | |
刊名 | CELLULAR & MOLECULAR IMMUNOLOGY |
2009 | |
卷号 | 6期号:3页码:159-165 |
关键词 | Pentylenetetrazol Brief seizures Morris water maze Contextual fear conditioning Acquisition Consolidation |
ISSN号 | 1672-7681 |
通讯作者 | zhengyt@mail.kiz.ac.cn |
英文摘要 | Dendritic cells (DCs) play a pivotal role in linking the innate immunity and acquired immunity in responses to pathogen. Non-human primates such as Chinese Rhesus Macaque (CRM) are the favorable models for preclinical study of potential therapeutic drugs, vaccines and mechanisms of human diseases. However, the phenotypical characterization of monocyte-derived dendritic cells (MDDCs) from CRM has not been elucidated. Monocytes from CRM were cultured with GM-CSF and IL-4 in RPMI-1640. Six days later, these cells were differentiated with typical dendritical morphology. CD11c and DC-SIGN were highly expressed. The immature MDDCs expressed the low levels of CD25, CD80, CD83, moderate CD40, CD86, and high MHC. After stimulation, the mature MDDCs increased expression of mature molecules CD25 and CD83, co-stimulatory molecules such as CD80, CD86 and CD40, and kept a high level of MHC. The capacity of endocytosis decreased with maturation. The mature MDDCs have strong ability of inducing allogeneic T cell proliferation and producing IL-12. In conclusion, we have characterized the phenotype and ultimate function of MDDCs from CRM for the first time. Cellular & Molecular Immunology. 2009;6(3):159-165. |
收录类别 | SCI |
资助信息 | This work was supported in part by grants from Scientific and Technological projects of China (2008ZX10001-002, 2008ZX10001-013, 2008ZX10005-005) and Yunnan (2006PT08), 973 Program (2006CB504208), the NSFC (30471605, 30671960, U0832601), and the CAS (KSCX1-YW-R-15) to Dr. YT Zheng, and “Western Light” Projects to Dr. GH Zhang. We also acknowledge Dr. Michael SC Tam for critical and constructive comments on the manuscript. |
语种 | 英语 |
公开日期 | 2010-08-24 |
内容类型 | 期刊论文 |
源URL | [http://159.226.149.42:8088/handle/152453/4665] |
专题 | 昆明动物研究所_分子免疫药理学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China |
推荐引用方式 GB/T 7714 | Xia HJ,Liu HL,Zhang GH,et al. Phenotype and Function of Monocyte-Derived Dendritic Cells from Chinese Rhesus Macaques[J]. CELLULAR & MOLECULAR IMMUNOLOGY,2009,6(3):159-165. |
APA | Xia HJ,Liu HL,Zhang GH,&Zheng YT[*].(2009).Phenotype and Function of Monocyte-Derived Dendritic Cells from Chinese Rhesus Macaques.CELLULAR & MOLECULAR IMMUNOLOGY,6(3),159-165. |
MLA | Xia HJ,et al."Phenotype and Function of Monocyte-Derived Dendritic Cells from Chinese Rhesus Macaques".CELLULAR & MOLECULAR IMMUNOLOGY 6.3(2009):159-165. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论