In vitro construction of a recombinant human embryonic brain-derived neurotrophin-4 gene and pEGFP-N1 vector

	In vitro construction of a recombinant human embryonic brain-derived neurotrophin-4 gene and pEGFP-N1 vector
	Li JT[*]1; Yan Q 2; Zhu XB 3; Xu D 1; Wang TH 1; Zhang HT 4; Liu J 1
刊名	中国神经再生研究（英文版）
	2009
卷号	4 期号:4 页码:312-315
关键词	human embryonic brain neurotrophin-4 gene construction in vitro
ISSN号	1673-5374
通讯作者	kmlijintao@163.com
合作状况	其它
英文摘要	BACKGROUND: Neurotrophin-4 (NT-4) can promote neuronal growth, development, differentiation, maturation, and survival. NT-4 can also improve recovery and regeneration of injured neurons, but cannot pass through the blood-brain barrier, which limits its activity in the central nervous system. Delivering NT-4 into the central nervous system via cells or vectors may have therapeutic benefit. OBJECTIVE: To construct a recombinant vector with a human embryonic brain-derived NT-4 gene and pEGFP-N1. DESIGN, TIME AND SETTING: Neural genetic engineering experiment. The study was performed at the Neuroscience Institute of Kunming Medical College between October 2007 and March 2008. MATERIALS: The pEGFP-N1 plasmid vector was provided by Kunming Institute of Zoology, Chinese Academy of Sciences; embryonic brain tissues were provided by the First Affiliated Hospital of Kunming Medical College. TRIzol RNA extraction Kit was purchased from Sigma (USA), One Step RNA PCR Kit (AMV) etc. were from Takara (Dalian, China). METHODS: Total RNA was extracted from human embryonic brain tissues using Trizol. The agarose gel electrophoresis showed two bands: 18 S and 28 S, which were essential subunits of total RNA. The human NT-4 DNA was obtained via RT-PCR and inserted into the pEGFP-N1 vector using ligation and transformation reaction. MAIN OUTCOME MEASURES: The sequencing results of the DNA in the recombinant of NT-4-pEGFP-N1. RESULTS: The NT-4-pEGFP-N1 vector was sequence-verified and showed the expected molecular weight. CONCLUSION: The recombinant of NT-4-pEGFP-N1 was constructed successfully in vitro.
收录类别	SCI
原文出处	200944312.pdf
语种	英语
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/4437]
专题	昆明动物研究所_分子免疫生物学
作者单位	1.Kunming Med Coll, Inst Neurosci, Kunming 650031, Yunnan, Peoples R China 2.Kunming Med Coll, Hosp 1, Dept Minimally Invas Neurosurg, Kunming 650032, Yunnan, Peoples R China 3.Chinese PLA, Kunming Gen Hosp Chengdu Mil Area Command, Dept Neurosurg, Kunming 650032, Yunnan, Peoples R China 4.Chinese Acad Sci, Kunming Inst Zool, Kunming 650223, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Li JT[*],Yan Q,Zhu XB,et al. In vitro construction of a recombinant human embryonic brain-derived neurotrophin-4 gene and pEGFP-N1 vector[J]. 中国神经再生研究（英文版）,2009,4(4):312-315.
APA	Li JT[*].,Yan Q.,Zhu XB.,Xu D.,Wang TH.,...&Liu J.(2009).In vitro construction of a recombinant human embryonic brain-derived neurotrophin-4 gene and pEGFP-N1 vector.中国神经再生研究（英文版）,4(4),312-315.
MLA	Li JT[*],et al."In vitro construction of a recombinant human embryonic brain-derived neurotrophin-4 gene and pEGFP-N1 vector".中国神经再生研究（英文版） 4.4(2009):312-315.