High-Efficiency Thermal Asymmetric Interlaced PCR (hiTAIL-PCR) for Determination of a Highly Degenerated Prophage WO Genome in a Wolbachia Strain Infecting a Fig Wasp Species

CORC > 昆明动物研究所 > 昆明动物研究所 > 遗传资源与进化国家重点实验室 > 分子进化基因组学

	High-Efficiency Thermal Asymmetric Interlaced PCR (hiTAIL-PCR) for Determination of a Highly Degenerated Prophage WO Genome in a Wolbachia Strain Infecting a Fig Wasp Species
	Wang GH 1,2; Xiao JH[]1; Xiong TL 1,2; Li Z 1,2; Murphy RW 3,4; Huang DW[]1,5
刊名	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
	2013
卷号	79 期号:23 页码:7476-7481
通讯作者	xiaojh@ioz.ac.cn ; huangdw@ioz.ac.cn
合作状况	其它
英文摘要	Temperate bacteriophage WO is a model system for studying tripartite interactions among viruses, bacteria, and eukaryotes, especially investigations of the genomic stability of obligate intracellular bacteria. Few WO genomes exist because of the difficulty in isolating viral DNA from eukaryotic hosts, and most reports are by-products of Wolbachia sequencing. Only one partial genome of a WO phage has been determined directly from isolated particles. We determine the complete genome sequence of prophage WO (WOSol) in Wolbachia strain wSol, which infects the fig wasp Ceratosolen solmsi (Hymenoptera: Chalcidoidea), by high-efficiency thermal asymmetric interlaced PCR. The genome of WOSol is highly degenerated and disrupted by a large region (14,267 bp) from Wolbachia. Consistent with previous molecular studies of multiple WO genomes, the genome of WOSol appears to have evolved by single nucleotide mutations and recombinations.
收录类别	SCI
资助信息	This project was supported by the National Natural Science Foundation of China (NSFC grants 31090253, 31172072, and 31210103912) and par- tially by the Major Innovation Program of Chinese Academy of Sciences (KSCX2-EW-Z-2), a grant from the Key Laboratory of the Zoological Systematics and Evolution of the Chinese Academy of Sciences (O529YX5105), and the National Science Fund for Fostering Talents in Basic Research (Special Subjects in Animal Taxonomy, NSFC-J0930004), and Discovery Grant 3148 from NSERC.
语种	英语
WOS记录号	WOS:000327544700041
公开日期	2014-01-03
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7764]
专题	昆明动物研究所_分子进化基因组学昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, China 2.University of Chinese Academy of Sciences, Beijing, China 3.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 4.Department of Natural History, Royal Ontario Museum, Toronto, Ontario, Canada 5.Plant Protection College, Shandong Agricultural University, Tai’an, China
推荐引用方式 GB/T 7714	Wang GH,Xiao JH[*],Xiong TL,et al. High-Efficiency Thermal Asymmetric Interlaced PCR (hiTAIL-PCR) for Determination of a Highly Degenerated Prophage WO Genome in a Wolbachia Strain Infecting a Fig Wasp Species[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2013,79(23):7476-7481.
APA	Wang GH,Xiao JH[],Xiong TL,Li Z,Murphy RW,&Huang DW[].(2013).High-Efficiency Thermal Asymmetric Interlaced PCR (hiTAIL-PCR) for Determination of a Highly Degenerated Prophage WO Genome in a Wolbachia Strain Infecting a Fig Wasp Species.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,79(23),7476-7481.
MLA	Wang GH,et al."High-Efficiency Thermal Asymmetric Interlaced PCR (hiTAIL-PCR) for Determination of a Highly Degenerated Prophage WO Genome in a Wolbachia Strain Infecting a Fig Wasp Species".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 79.23(2013):7476-7481.