Potential pitfalls in MitoChip detected tumor-specific somatic mutations: a call for caution when interpreting patient data

CORC > 昆明动物研究所 > 昆明动物研究所 > 遗传资源与进化国家重点实验室 > 分子进化基因组学

	Potential pitfalls in MitoChip detected tumor-specific somatic mutations: a call for caution when interpreting patient data
	Palanichamy MG[]1,2; Zhang YP[]1,2
刊名	BMC CANCER
	2010
卷号	10 期号:X 页码:e597
通讯作者	empalani@yahoo.com ; zhangyp1@263.net.cn
合作状况	其它
英文摘要	Background: Several investigators have employed high throughput mitochondrial sequencing array (MitoChip) in clinical studies to search mtDNA for markers linked to cancers. In consequence, a host of somatic mtDNA mutations have been identified as linked to different types of cancers. However, closer examination of these data show that there are a number of potential pitfalls in the detection tumor-specific somatic mutations in clinical case studies, thus urging caution in the interpretation of mtDNA data to the patients. This study examined mitochondrial sequence variants demonstrated in cancer patients, and assessed the reliability of using detected patterns of polymorphisms in the early diagnosis of cancer. Methods: Published entire mitochondrial genomes from head and neck, adenoid cystic carcinoma, sessile serrated adenoma, and lung primary tumor from clinical patients were examined in a phylogenetic context and compared with known, naturally occurring mutations which characterize different populations. Results: The phylogenetic linkage analysis of whole arrays of mtDNA mutations from patient cancerous and non-cancerous tissue confirmed that artificial recombination events occurred in studies of head and neck, adenoid cystic carcinoma, sessile serrated adenoma, and lung primary tumor. Our phylogenetic analysis of these tumor and control leukocyte mtDNA haplotype sequences shows clear cut evidence of mixed ancestries found in single individuals. Conclusions: Our study makes two prescriptions: both in the clinical situation and in research 1. more care should be taken in maintaining sample identity and 2. analysis should always be undertaken with respect to all the data available and within an evolutionary framework to eliminate artifacts and mix-ups.
收录类别	SCI
语种	英语
公开日期	2010-12-17
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/353002/6547]
专题	昆明动物研究所_分子进化基因组学昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, 2 North Green Lake Street, Kunming 650091, China 2.State Key Laboratory of Genetic resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
推荐引用方式 GB/T 7714	Palanichamy MG[],Zhang YP[]. Potential pitfalls in MitoChip detected tumor-specific somatic mutations: a call for caution when interpreting patient data[J]. BMC CANCER,2010,10(X):e597.
APA	Palanichamy MG[],&Zhang YP[].(2010).Potential pitfalls in MitoChip detected tumor-specific somatic mutations: a call for caution when interpreting patient data.BMC CANCER,10(X),e597.
MLA	Palanichamy MG[*],et al."Potential pitfalls in MitoChip detected tumor-specific somatic mutations: a call for caution when interpreting patient data".BMC CANCER 10.X(2010):e597.