Adaptive evolution of a duplicated pancreatic ribonuclease gene in a leaf-eating monkey

CORC > 昆明动物研究所 > 昆明动物研究所 > 遗传资源与进化国家重点实验室 > 分子进化基因组学

	Adaptive evolution of a duplicated pancreatic ribonuclease gene in a leaf-eating monkey
	Zhang JZ[*]1,2; Zhang YP 3; Rosenberg HF 1
刊名	NATURE GENETICS
	2002
卷号	30 期号:4 页码:411-415
ISSN号	1061-4036
通讯作者	jianzhi@umich.edu
合作状况	其它
英文摘要	Although the complete genome sequences of over 50 representative species have revealed the many duplicated genes in all three domains of life(1-4), the roles of gene duplication in organismal adaptation and biodiversity are poorly understood. In addition, the evolutionary forces behind the functional divergence of duplicated genes are often unknown, leading to disagreement on the relative importance of positive Darwinian selection versus relaxation of functional constraints in this process(5-10). The methodology of earlier studies relied largely on DNA sequence analysis but lacked functional assays of duplicated genes, frequently generating contentious results(11,12). Here we use both computational and experimental approaches to address these questions in a study of the pancreatic ribonuclease gene (RNASE1) and its duplicate gene (RNASE1B) in a leaf-eating colobine monkey, douc langur. We show that RNASE1B has evolved rapidly under positive selection for enhanced ribonucleolytic activity in an altered microenvironment, a response to increased demands for the enzyme for digesting bacterial RNA. At the same time, the ability to degrade double-stranded RNA, a non-digestive activity characteristic of primate RNASE1, has been lost in RNASE1B, indicating functional specialization and relaxation of purifying selection. Our findings demonstrate the contribution of gene duplication to organismal adaptation and show the power of combining sequence analysis and functional assays in delineating the molecular basis of adaptive evolution.
收录类别	SCI
资助信息	This work was supported in part by a start-up fund and a Rackham grant from the University of Michigan (to J.Z.) and grants from the Natural Science Foundation of China and Chinese Academy of Sciences (to Y.P.Z.).
原文出处	200230411.pdf
语种	英语
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/4067]
专题	昆明动物研究所_分子进化基因组学
作者单位	1.Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA 2.Departments of Ecology and Evolutionary Biology and Molecular, Cellular and Developmental Biology, University of Michigan, 3003 Natural Sciences Building, 830 North University Avenue, Ann Arbor, Michigan 48109, USA 3.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China
推荐引用方式 GB/T 7714	Zhang JZ[*],Zhang YP,Rosenberg HF. Adaptive evolution of a duplicated pancreatic ribonuclease gene in a leaf-eating monkey[J]. NATURE GENETICS,2002,30(4):411-415.
APA	Zhang JZ[*],Zhang YP,&Rosenberg HF.(2002).Adaptive evolution of a duplicated pancreatic ribonuclease gene in a leaf-eating monkey.NATURE GENETICS,30(4),411-415.
MLA	Zhang JZ[*],et al."Adaptive evolution of a duplicated pancreatic ribonuclease gene in a leaf-eating monkey".NATURE GENETICS 30.4(2002):411-415.