Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact

	Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact
	Zhang C 1,2; Zhang HY 1,2,3; Yu L 4; Cao Y[*]1
刊名	HELICOBACTER
	2014
卷号	19 期号:5 页码:330-342
关键词	Helicobacter pylori gastrointestinal epithelium tight junctions adherens junctions MUC1
通讯作者	caoy@mail.kiz.ac.cn
合作状况	其它
英文摘要	BackgroundEpithelial junctions and mucins compose a major portion of the mucosal barrier. Helicobacter pylori (H.pylori) infections induce alterations of the tight junctions and adherens junctions in epithelial cells, although the precise mechanisms underlying this process are not fully understood. MethodsThe expression of adhesion molecules and MUC1 was systematically investigated in gastrointestinal epithelial cells infected with H.pylori in vitro and in vivo. Furthermore, we developed several new in vitro methods to study the relationships between the bacterium and the dysfunction of tight junctions using Boyden Chambers. ResultsThe expression of a series of junctional molecules and MUC1 decreased in the cultured cells that were infected with H.pylori. According to the degree of damage at the tight junctions, direct contact of H.pylori with the apical membrane of the cells resulted in the greatest increase in permeability compared to basal membrane binding or non-binding of H.pylori to the cells. Similarly, we noted that H.pylori infection could reduce the expression and glycosylation of MUC1. ConclusionsHelicobacter pylori dwelling on the apical surface of the gastrointestinal epithelium could directly induce serious injury of the mucosal barrier, and the new methods outlined here, based on the Boyden Chamber system, could be very useful for studying the relationships between bacteria and their target cells.
收录类别	SCI
资助信息	The study was supported by National Natural Science Founda- tion of China (No. 30800407 and 81201667), 100 Talents Program of Chinese Academy of Sciences (Grant Cao), and Science and Technology Department, Yunnan Province, China (Y103951111).
语种	英语
WOS记录号	WOS:000342989000002
公开日期	2014-11-04
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/8160]
专题	昆明动物研究所_分子病理学
作者单位	1.Laboratory of Molecular and Experimental Pathology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 2.Kunming College of Life Science, University of the Chinese Academy of Sciences, Kunming, China 3.Department of Gastroenterology, Kunming First People Hospital, Kunming, China 4.Department of Pathology, Kunming First People Hospital, Kunming, China
推荐引用方式 GB/T 7714	Zhang C,Zhang HY,Yu L,et al. Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact[J]. HELICOBACTER,2014,19(5):330-342.
APA	Zhang C,Zhang HY,Yu L,&Cao Y[*].(2014).Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact.HELICOBACTER,19(5),330-342.
MLA	Zhang C,et al."Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact".HELICOBACTER 19.5(2014):330-342.