Suppression subtractive hybridization identified differentially expressed genes in lung adenocarcinoma: ERGIC3 as a novel lung cancer-related gene

	Suppression subtractive hybridization identified differentially expressed genes in lung adenocarcinoma: ERGIC3 as a novel lung cancer-related gene
	Wu MS 1,2,3; Tu T 1,4; Huang YC 5; Cao Y[*]1
刊名	BMC CANCER
	2013
卷号	13 期号:X 页码:e44
关键词	Lung cancer cDNA library Suppression subtractive hybridization ERGIC3 Erv46
通讯作者	caoy@mail.kiz.ac.cn
合作状况	其它
英文摘要	Background: To understand the carcinogenesis caused by accumulated genetic and epigenetic alterations and seek novel biomarkers for various cancers, studying differentially expressed genes between cancerous and normal tissues is crucial. In the study, two cDNA libraries of lung cancer were constructed and screened for identification of differentially expressed genes. Methods: Two cDNA libraries of differentially expressed genes were constructed using lung adenocarcinoma tissue and adjacent nonmalignant lung tissue by suppression subtractive hybridization. The data of the cDNA libraries were then analyzed and compared using bioinformatics analysis. Levels of mRNA and protein were measured by quantitative real-time polymerase chain reaction (q-RT-PCR) and western blot respectively, as well as expression and localization of proteins were determined by immunostaining. Gene functions were investigated using proliferation and migration assays after gene silencing and gene over-expression. Results: Two libraries of differentially expressed genes were obtained. The forward-subtracted library (FSL) and the reverse-subtracted library (RSL) contained 177 and 59 genes, respectively. Bioinformatic analysis demonstrated that these genes were involved in a wide range of cellular functions. The vast majority of these genes were newly identified to be abnormally expressed in lung cancer. In the first stage of the screening for 16 genes, we compared lung cancer tissues with their adjacent non-malignant tissues at the mRNA level, and found six genes (ERGIC3, DDR1, HSP90B1, SDC1, RPSA, and LPCAT1) from the FSL were significantly up-regulated while two genes (GPX3 and TIMP3) from the RSL were significantly down-regulated (P < 0.05). The ERGIC3 protein was also over-expressed in lung cancer tissues and cultured cells, and expression of ERGIC3 was correlated with the differentiated degree and histological type of lung cancer. The up-regulation of ERGIC3 could promote cellular migration and proliferation in vitro. Conclusions: The two libraries of differentially expressed genes may provide the basis for new insights or clues for finding novel lung cancer-related genes; several genes were newly found in lung cancer with ERGIC3 seeming a novel lung cancer-related gene. ERGIC3 may play an active role in the development and progression of lung cancer.
收录类别	SCI
资助信息	This work was supported by 973 Program (2011CB510104), Natural Science Foundation of China (81272617), and a grant from the Yunnan Province Science and Technology Department (Y103951111). We thank Dr. G-F Li and Dr. Y Lei (Tumor Hospital of Yunnan Province) for providing part of patients’ samples, and K-D Zhang (Kunming Institute of Zoology) for preparing part of experiments.
语种	英语
WOS记录号	WOS:000314585400001
公开日期	2013-03-07
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7267]
专题	昆明动物研究所_分子病理学昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanism, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Graduate University of the Chinese Academy of Sciences, Beijing 100049, China 3.Department of Cell Biology and Genetics, Zunyi Medical College, Zunyi 563003, China 4.Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Chin 5.Department of Thoracic Surgery, Tumor Hospital of Yunnan Province, Kunming 650106, China.
推荐引用方式 GB/T 7714	Wu MS,Tu T,Huang YC,et al. Suppression subtractive hybridization identified differentially expressed genes in lung adenocarcinoma: ERGIC3 as a novel lung cancer-related gene[J]. BMC CANCER,2013,13(X):e44.
APA	Wu MS,Tu T,Huang YC,&Cao Y[*].(2013).Suppression subtractive hybridization identified differentially expressed genes in lung adenocarcinoma: ERGIC3 as a novel lung cancer-related gene.BMC CANCER,13(X),e44.
MLA	Wu MS,et al."Suppression subtractive hybridization identified differentially expressed genes in lung adenocarcinoma: ERGIC3 as a novel lung cancer-related gene".BMC CANCER 13.X(2013):e44.