Expression and distribution of symplekin regulates the assembly and function of the epithelial tight junction

	Expression and distribution of symplekin regulates the assembly and function of the epithelial tight junction
	Chang H 1,2; Zhang C 1,2; Cao Y[*]1
刊名	HISTOCHEMISTRY AND CELL BIOLOGY
	2012
卷号	137 期号:3 页码:319-327
关键词	Symplekin Tight junction Permeability Polarity ZO-1
通讯作者	caoy@mail.kiz.ac.cn
合作状况	其它
英文摘要	Symplekin is multifunctional protein localized to both the tight junction and the nucleus with known roles in mRNA polyadenylation, proliferation, differentiation and tumorigenesis. Functions of symplekin at tight junctions have not been systematically investigated. In this study, increased expression of symplekin was observed during the formation of tight junctions in cultured HT-29 and HepG2 epithelial cells. Repression of symplekin by RNAi increased the permeability of epithelial monolayers, disrupted cellular polarity, and decreased the expression of the tight junction protein ZO-1. Moreover, symplekin was co-localized with ZO-1 at tight junctions and co-immunoprecipitated with ZO-1, indicating that ZO-1 and symplekin form complexes. In conclusion, symplekin expression regulates the assembly of tight junctions, thus helps to maintain the integrity of the epithelial monolayer and cellular polarity
收录类别	SCI
资助信息	This study was supported by National Natural Science Foundation of China (No. 30800407) and Human Talents Pro- gram of Chinese Academy of Sciences (Grant Cao).
语种	英语
WOS记录号	WOS:000300326100005
公开日期	2012-03-19
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/353002/6875]
专题	昆明动物研究所_分子病理学昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanism of CAS and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming, Yunnan 650223, Peoples’ Republic of China 2.Graduate University of the Chinese Academy of Sciences, Beijing 100049, China
推荐引用方式 GB/T 7714	Chang H,Zhang C,Cao Y[*]. Expression and distribution of symplekin regulates the assembly and function of the epithelial tight junction[J]. HISTOCHEMISTRY AND CELL BIOLOGY,2012,137(3):319-327.
APA	Chang H,Zhang C,&Cao Y[*].(2012).Expression and distribution of symplekin regulates the assembly and function of the epithelial tight junction.HISTOCHEMISTRY AND CELL BIOLOGY,137(3),319-327.
MLA	Chang H,et al."Expression and distribution of symplekin regulates the assembly and function of the epithelial tight junction".HISTOCHEMISTRY AND CELL BIOLOGY 137.3(2012):319-327.