Methylation of tumor associated genes in tissue and plasma samples from liver disease patients

	Methylation of tumor associated genes in tissue and plasma samples from liver disease patients
	Chang H 1,5; Yi B 1,5; Li L 2,5; Zhang HY 3; Sun F 4; Dong SQ 3; Cao Y[*]1,5
刊名	EXPERIMENTAL AND MOLECULAR PATHOLOGY
	2008
卷号	85 期号:2 页码:96-100
关键词	Hepatocellular carcinoma Cirrhosis Plasma DNA Hypermethylation Methylation-specific PCR
ISSN号	0014-4800
通讯作者	caoy@mail.kiz.ac.cn
英文摘要	To investigate whether aberrant hypermethylation in plasma DNA could be used as diagnosis makers for hepatocellular carcinoma (HCC), we performed methylation-specific PCR (MSP) to check the methylation status of five tumor associated genes in 36 cases of tissue and 42 cases of plasma samples from HCC and liver cirrhosis patients, respectively. The hypermethylation frequency of GSTP1 and RASSF1A showed significant difference between HCCs and liver cirrhosis with OF without HBV infection (P < 0.05), but differences of the hypermethylation status of APC, E-cadherin, and P16 were not statistically significant. There were no significant differences in the hypermethylation status of five genes between the groups of cirrhosis with and without HBV infection. The significant differences of E-cadherin, GSTP1, P16, and RASSF1A in methylation between HCCs and liver cirrhosis were not observed in the plasma samples. Furthermore, the inconsistent results of MSP and real-time quantitative PCR for the paired samples of tissue and plasma suggested that plasma DNA could not fully stand for tissue DNA. In conclusion, hypermethylation of some specific, but not all, tumor associated genes may be involved in hepatocarcinogenesis; examination of the methylation status of E-cadherin, GSTP1, P16, and RASSF1A in the plasma samples might have limited usage for HCC diagnosis.
收录类别	SCI
资助信息	This study was supported by a grant from Yunnan Province Science and Technology Department (2007C097 M).
语种	英语
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/3369]
专题	昆明动物研究所_分子病理学昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Laboratory of Molecular and Experimental Pathology, Key Laboratory of Animal Models and Human Disease Mechanism, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 2.Department of General Surgery, Kunming First People Hospital, Kunming, China 3.Division of Digestive Diseases, Kunming First People Hospital, Kunming, China 4.Surgical Department of Hepato-Bilio-Pancreatology, Second Affiliated Hospital of Kunming Medical Collage, Kunming, China 5.Yunnan Liver Diseases Center, Kunming, China
推荐引用方式 GB/T 7714	Chang H,Yi B,Li L,et al. Methylation of tumor associated genes in tissue and plasma samples from liver disease patients[J]. EXPERIMENTAL AND MOLECULAR PATHOLOGY,2008,85(2):96-100.
APA	Chang H.,Yi B.,Li L.,Zhang HY.,Sun F.,...&Cao Y[*].(2008).Methylation of tumor associated genes in tissue and plasma samples from liver disease patients.EXPERIMENTAL AND MOLECULAR PATHOLOGY,85(2),96-100.
MLA	Chang H,et al."Methylation of tumor associated genes in tissue and plasma samples from liver disease patients".EXPERIMENTAL AND MOLECULAR PATHOLOGY 85.2(2008):96-100.