The Ubiquitin Ligase RNF220 Enhances Canonical Wnt Signaling through USP7-Mediated Deubiquitination of beta-Catenin

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	The Ubiquitin Ligase RNF220 Enhances Canonical Wnt Signaling through USP7-Mediated Deubiquitination of beta-Catenin
	Ma PC 1; Yang XC 1,2; Kong QH 1; Li CC 1; Yang SJ 3; Li Y 4; Mao BY[*]1
刊名	MOLECULAR AND CELLULAR BIOLOGY
	2014
卷号	34 期号:23 页码:4355-4366
通讯作者	mao@mail.kiz.ac.cn
合作状况	其它
英文摘要	Wnt/beta-catenin signaling plays critical roles in embryonic development and disease. Here, we identify RNF220, a RING domain E3 ubiquitin ligase, as a new regulator of beta-catenin. RNF220 physically interacts with beta-catenin, but instead of promoting its ubiquitination and proteasomal degradation, it stabilizes beta-catenin and promotes canonical Wnt signaling. Our analysis showed that RNF220 interacts with USP7, a ubiquitin-specific peptidase, which is required for RNF220 to stabilize beta-catenin. The RNF220/USP7 complex deubiquitinates beta-catenin and enhances canonical Wnt signaling. Interestingly, the stability of RNF220 itself is negatively regulated by Gsk3 beta, which is a key component of the beta-catenin destruction complex and is inhibited upon Wnt stimulation. Accordingly, the RNF220/USP7 complex works as a positive feedback regulator of beta-catenin signaling. In colon cancer cells with stimulated Wnt signaling, knockdown of RNF220 or USP7 impairs Wnt signaling and expression of Wnt target genes, suggesting a potentially novel role of RNF220 in Wnt-related tumorigenesis.
收录类别	SCI
资助信息	This work was supported by grants from the National Natural Science Foundation of China (31171404) and West Light Foundation of the Chi- nese Academy of Sciences.
语种	英语
WOS记录号	WOS:000344631500011
公开日期	2014-12-05
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/8187]
专题	昆明动物研究所_发育生物学昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology 2.Kunming College of Life Science, University of Chinese Academy of Sciences 3.Kunming Biological Diversity Regional Center of Large Apparatus and Equipments, Kunming Institute of Zoology 4.State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, d Kunming, China
推荐引用方式 GB/T 7714	Ma PC,Yang XC,Kong QH,et al. The Ubiquitin Ligase RNF220 Enhances Canonical Wnt Signaling through USP7-Mediated Deubiquitination of beta-Catenin[J]. MOLECULAR AND CELLULAR BIOLOGY,2014,34(23):4355-4366.
APA	Ma PC.,Yang XC.,Kong QH.,Li CC.,Yang SJ.,...&Mao BY[*].(2014).The Ubiquitin Ligase RNF220 Enhances Canonical Wnt Signaling through USP7-Mediated Deubiquitination of beta-Catenin.MOLECULAR AND CELLULAR BIOLOGY,34(23),4355-4366.
MLA	Ma PC,et al."The Ubiquitin Ligase RNF220 Enhances Canonical Wnt Signaling through USP7-Mediated Deubiquitination of beta-Catenin".MOLECULAR AND CELLULAR BIOLOGY 34.23(2014):4355-4366.