Xenopus Nkx6.1 and Nkx6.2 are required for mid-hindbrain boundary development

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	Xenopus Nkx6.1 and Nkx6.2 are required for mid-hindbrain boundary development
	Ma PC 1,2; Xia YJ 1,2; Ma L 1; Zhao SH[]3; Mao BY[]1,4
刊名	DEVELOPMENT GENES AND EVOLUTION
	2013
卷号	223 期号:4 页码:253-259
关键词	nkx6.1 nkx6.2 mid-hindbrain boundary wnt1 Xenopus
通讯作者	sowardzhao@gmail.com ; mao@mail.kiz.ac.cn
合作状况	其它
英文摘要	The mid-hindbrain boundary (MHB) organizer is among the best studied local organizers that patterns the midbrain and cerebellum in vertebrates and is established by a regulatory network of several transcription factors and signals, including Wnt signaling. In the present study, we found that Xenopus Nkx6.1 and Nkx6.2, two transcription factors of the Nkx homeobox family, are required for MHB formation. In Xenopus embryos, nkx6.1 and nkx6.2 are expressed in the MHB, and knockdown of either nkx6.1 or nkx6.2 by specific morpholinos disrupts the MHB expression of en2 as well as wnt1, a key regulator for vertebrate MHB formation. In the nkx6.1/nkx6.2 morphants, co-injection of wnt1 or dngsk3 beta mRNA, which activates Wnt signaling, rescues the expression of en2. Nkx6.1 and Nkx6.2 activate canonical Wnt signaling in reporter assays in both cultured mammalian cells and Xenopus embryos. An Nkx6.2 deletion construct, which inhibits the ability of wild type Nkx6.2 to activate Wnt signaling, also reduces the MHB expression of en2 in Xenopus embryos. These results suggest that Nkx6.1 and Nkx6.2 are involved in MHB formation in Xenopus embryos, likely by modulating wnt1 expression.
收录类别	SCI
资助信息	This work was supported by grants from the National Nat- ural Science Foundation of China (90919039 and 31171404).
语种	英语
WOS记录号	WOS:000320671600005
公开日期	2013-08-08
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7577]
专题	昆明动物研究所_发育生物学昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, People’s Republic of China 2.University of the Chinese Academy of Sciences, Beijing 100049, People’s Republic of China 3.Yunnan Key Laboratory of Fertility Regulation and Minority Eugenics, Yunnan Population and Family Planning Research Institute, Kunming 650021, People’s Republic of China 4.Kunming Institute of Zoology, 32 East Jiao Chang Road, Kunming, Yunnan 650223, People’s Republic of China
推荐引用方式 GB/T 7714	Ma PC,Xia YJ,Ma L,et al. Xenopus Nkx6.1 and Nkx6.2 are required for mid-hindbrain boundary development[J]. DEVELOPMENT GENES AND EVOLUTION,2013,223(4):253-259.
APA	Ma PC,Xia YJ,Ma L,Zhao SH[],&Mao BY[].(2013).Xenopus Nkx6.1 and Nkx6.2 are required for mid-hindbrain boundary development.DEVELOPMENT GENES AND EVOLUTION,223(4),253-259.
MLA	Ma PC,et al."Xenopus Nkx6.1 and Nkx6.2 are required for mid-hindbrain boundary development".DEVELOPMENT GENES AND EVOLUTION 223.4(2013):253-259.