RNF220, an E3 ubiquitin ligase that targets Sin3B for ubiquitination

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	RNF220, an E3 ubiquitin ligase that targets Sin3B for ubiquitination
	Kong QH 1,2; Zeng WL 1,2; Wu JY 1,2; Hu WL 1,2; Li CC 1; Mao BY[*]1
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2010
卷号	393 期号:4 页码:708-713
关键词	RNF220 Sin3B E3 ligase Ubiquitination Degradation
ISSN号	0006-291X
通讯作者	mao@mail.kiz.ac.cn
合作状况	其它
英文摘要	Modification of proteins by ubiquitination plays important roles in various cellular processes. During this process, the target specificity is determined by ubiquitin ligases. Here we identify RNF220 (RING finger protein 220) as a novel ubiquitin ligase for Sin3B. As a conserved RING protein, RNF220 can bind E2 and mediate auto-ubiquitination of itself. Through a yeast two-hybrid screen, we isolated Sin3B as one of its targets, which is a scaffold protein of the Sin3/HDAC (histone deacetylase) corepressor complex. RNF220 specifically interacts with Sin3B both in vitro and in vivo. Sin3B can be regulated by the ubiquitin-proteasome system. Co-expression of RNF220 promotes the ubiquitination and proteasomal degradation of Sin3B. Taken together, these results reveal a new mechanism for regulating the Sin3/HDAC complex.
收录类别	SCI
资助信息	This work was supported by grants from the Key State Research Program from the Ministry of Science and Technology of China (2006CB943902 and 2007CB947201), the National Natural Science Foundation of China (30425011 and 305303800), and the Innova- tion Project of Chinese Academy of Sciences (KSCX2-YW-R-090).
语种	英语
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/3171]
专题	昆明动物研究所_发育生物学昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.CAS-Max Planck Junior Scientist Group on Developmental Biology, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang East Road, Kunming 650223, China 2.Graduate University of Chinese Academy of Sciences, Beijing 100049, China
推荐引用方式 GB/T 7714	Kong QH,Zeng WL,Wu JY,et al. RNF220, an E3 ubiquitin ligase that targets Sin3B for ubiquitination[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2010,393(4):708-713.
APA	Kong QH,Zeng WL,Wu JY,Hu WL,Li CC,&Mao BY[*].(2010).RNF220, an E3 ubiquitin ligase that targets Sin3B for ubiquitination.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,393(4),708-713.
MLA	Kong QH,et al."RNF220, an E3 ubiquitin ligase that targets Sin3B for ubiquitination".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 393.4(2010):708-713.