中华大蟾蜍皮肤Cathelicidin家族新型抗菌肽的鉴定及其抗菌活性

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 动物活性蛋白多肽组学

	中华大蟾蜍皮肤Cathelicidin家族新型抗菌肽的鉴定及其抗菌活性
	展波 1; 高媛媛 2; 李文辉[*]1,3; 林维平 1; 贾广韬[1; 孙同毅 1
刊名	中国中药杂志
	2016
卷号	41 期号:4 页码:630-635
关键词	中华大蟾蜍 Cathelicidin 抗菌肽转录组嗜水气单胞菌
其他题名	Identification and bactericidal activity of a novel Cathelicidin family member from skin of Bufu bufo gargarizans
通讯作者	leewh@mail.kiz.ac.cn
合作状况	其它
中文摘要	对中华大蟾蜍皮肤转录组进行了测定,通过对得到转录组数据的组装、注释和序列比对得到1个新型Cathelicidin家族前体c DNA。按照Cathelicidin家族抗菌肽的加工方式,推导出该前体可能编码2个成熟的中华大蟾蜍Cathelicidin家族抗菌肽,命名为BG-CATH25,BG-CATH29。圆二色散测定了合成BG-CATH25,BG-CATH29的二级结构特征,结果揭示BG-CATH25,BG-CATH29在不同缓冲体系中以无规则卷曲的构象为主。对实验所用4种细菌的抗菌活性研究表明BG-CATH25,BGCATH29对嗜水气单胞菌有较强的抑菌活性,MIC分别为1.25,10 mg·L-1。
英文摘要	The skin transcriptome of Bufu bufo gargarizans was determined by conventional methods. A novel full length c DNA coding for a Cathelicidin precursor was identified by transcriptomic data assembling,annotation and blast search of corresponding data banks. According to the known processing methods of Cathelicidin family members,present reported novel Cathelicidin precursor of B. bufo gargarizans might be cleaved at 2 possible sites of the same precursor and generate both BG-CATH25 and BG-CATH29 as mature molecules. The deduced BG-CATH25 and BG-CATH29 were synthesized with purity 95% to evaluate the properties and bactericidal activities. The secondary structural characteristics of both BG-CATH25 and BG-CATH29 in different solutions were determined by Circular Dichroism（ CD） Analysis. CD results indicated that random coil conformation were the main structural elements for both BGCATH25 and BGCATH29 in different buffer systems. Antimicrobial activities against tested bacterial strains were carried out by plating method. Both BG-CATH25 and BG-CATH29 showed strong antibacterial activities against Aeromonas hydrophila,with MIC values of 1. 25,10mg·L- 1,respectively. However,both of them showed weak bactericidal activities against human pathogenic bacteria,like Escherichia coli（ ATCC25922）,Staphylococcus aureus（ ATCC25923） and Pseudomonas aeruginosa（ ATCC 27853）.
收录类别	其他
资助信息	国家自然科学基金项目(81102363)；山东省优秀中青年科学家科研奖励基金项目(bs2012yy037,bs2013yy063)；山东省自然科学基金项目(zr2015hl056)；山东省高等学校科技计划项目(j15lm10)
语种	中文
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10254]
专题	昆明动物研究所_动物活性蛋白多肽组学昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.潍坊医学院生物科学与技术学院,山东潍坊261053 2.潍坊医学院临床医学院,山东潍坊261053 3.中国科学院昆明动物研究所,云南省动物模型与人类疾病机理重点实验室,云南昆明650223
推荐引用方式 GB/T 7714	展波,高媛媛,李文辉[*],等. 中华大蟾蜍皮肤Cathelicidin家族新型抗菌肽的鉴定及其抗菌活性[J]. 中国中药杂志,2016,41(4):630-635.
APA	展波,高媛媛,李文辉[*],林维平,贾广韬[,&孙同毅.(2016).中华大蟾蜍皮肤Cathelicidin家族新型抗菌肽的鉴定及其抗菌活性.中国中药杂志,41(4),630-635.
MLA	展波,et al."中华大蟾蜍皮肤Cathelicidin家族新型抗菌肽的鉴定及其抗菌活性".中国中药杂志 41.4(2016):630-635.