Acute toxicity of betagamma-CAT, a naturally existing non-lens betagamma-crystallin and trefoil factor complex from frog Bombina maxima skin secretions

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 动物活性蛋白多肽组学

	Acute toxicity of betagamma-CAT, a naturally existing non-lens betagamma-crystallin and trefoil factor complex from frog Bombina maxima skin secretions
	Qian JQ 1,2,3; Liu SB 1,3; He YY 1,3; Lee WH 1; Zhang Y[*]1
刊名	TOXICON
	2008
卷号	52 期号:1 页码:22-31
关键词	non-lens beta gamma-crystallin trefoil factor amphibian beta gamma-CAT toxicity cardiovascular collapse
通讯作者	zhangy@mail.kiz.ac.cn
合作状况	其它
英文摘要	In vertebrates, non-lens beta gamma-crystallins are widely expressed in various tissues, but their functions are unknown. The molecular mechanisms of trefoil factors, initiators of mucosal healing and being greatly involved in tumorigenesis, have remained elusive. beta gamma-CAT is the first example of a naturally existing multifunctional protein complex of a non-lens beta gamma-crystallin and a trefoil factor from frog Bombina maxima skin secretions. Here we report the investigation of its in vivo toxic effects on mice and rabbits. The LD(50) values of beta gamma-CAT on mice were determined to be 0.4 mg/kg and 20 mu g/kg under intraperitoneal (i.p.) and intravenous (i.v.) injection, respectively. The mice subcutaneously injected with beta gamma-CAT (6 mu g/g body weight) showed strong hyperaemia of subcutaneous capillary vessel, but no hemorrhagic spots were observed. Intravenous injection of beta gamma-CAT in rabbits (8-22 mu g/kg body weight) caused a rapidly hypotensive effect and followed with cardiovascular collapse. Injection with beta gamma-CAT (22 mu g/kg, i.v.) significantly decreased hematocrit (P < 0.05) and mean corpuscular volume (P < 0.05) of the rabbits in 5 min. At the same time, the counts of platelets and white blood cells were significantly decreased (P < 0.05), while the blood levels of glucose, lactate dehydrogenase and serum glutamic-oxaloacetic transaminase were significantly increased (P < 0.05). Furthermore, serials of tissues edema and damages were also observed. These results indicate that beta gamma-CAT rapidly caused several in vivo toxic effects on mammals and its lethal toxic potency was mainly contributed by hypotension and cardiovascular collapse, providing new clues for the understanding of the patho-physiological roles of non-lens beta gamma-crystallins and trefoil factors.
收录类别	SCI
资助信息	This work was supported by grants from The Chinese National Natural Science Foundation (30630014 and 30570359), and grants of ‘‘Key Research Direction-KSCX2- YW-R-088’’ from The Chinese Academy of Sciences and The Yunnan Science and Technology Commission (2006PT08-1).
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/9625]
专题	昆明动物研究所_动物活性蛋白多肽组学昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, PR China 2.Department of Anesthesiology, The First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan, PR China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100039, PR China
推荐引用方式 GB/T 7714	Qian JQ,Liu SB,He YY,et al. Acute toxicity of betagamma-CAT, a naturally existing non-lens betagamma-crystallin and trefoil factor complex from frog Bombina maxima skin secretions[J]. TOXICON,2008,52(1):22-31.
APA	Qian JQ,Liu SB,He YY,Lee WH,&Zhang Y[*].(2008).Acute toxicity of betagamma-CAT, a naturally existing non-lens betagamma-crystallin and trefoil factor complex from frog Bombina maxima skin secretions.TOXICON,52(1),22-31.
MLA	Qian JQ,et al."Acute toxicity of betagamma-CAT, a naturally existing non-lens betagamma-crystallin and trefoil factor complex from frog Bombina maxima skin secretions".TOXICON 52.1(2008):22-31.