A novel annexin A2 protein with platelet aggregation-inhibiting activity from amphibian Bombina maxima skin

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 动物活性蛋白多肽组学

	A novel annexin A2 protein with platelet aggregation-inhibiting activity from amphibian Bombina maxima skin
	Zhang Y 1,2; Yu GY 1,2,3; Lee WH 1; Zhang Y[*]1; Wang YJ 1,2; Zhang J 1,2; Wei SS 1,2
刊名	TOXICON
	2010
卷号	56 期号:3 页码:458-465
关键词	Amphibian Skin Annexin Platelet activation
通讯作者	zhangy@mail.kiz.ac.cn
合作状况	其它
英文摘要	Annexin A2 is a unique member of annexin family with multi-functions in membrane physiology, implicated in inflammation and cancer progression. mRNA of Annexin A2 is abundant in the skin of some amphibians. However, no annexin A2 protein has been isolated and characterized from amphibian skin. In this report, a novel annexin A2 protein with apparent molecular weight of 33 kDa and named Bm-ANXA2, was purified from frog Bombina maxima skin, which is highly toxic to mammals, by a combination of ion exchange and gel filtration chromatography. A full-length cDNA encoding the protein was obtained from the cDNA library constructed from the frog skin. Sequence analysis indicates that Bm-ANXA2 shares 89% and 80% amino acid sequence identities with those of Xenopus and human annexin A2, respectively. Different from other annexin A2 proteins, the N-terminal 26 amino acids of Bm-ANXA2 were truncated. Bm-ANXA2 dose-dependently inhibited human platelet aggregation stimulated by various agonists in a Ca2+-dependent manner. It bound to activated platelets and significantly inhibited alpha(IIb)beta(3) activation and alpha-granular secretion. This is the first report that an annexin A2 protein possesses platelet aggregation-inhibiting activity, providing novel clues in the illustration of pathophysiological roles of annexin A2 proteins.
收录类别	SCI
资助信息	This work was supported by grants from National Basic Research Program of China (973 Program, 2010CB529800), the Chinese National Natural Science Foundation (30630014 and 30870304), and the Chinese Academy of Sciences “Key Research Direction” (KSCX2-YW- R-088) to Yun Zhang.
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/9610]
专题	昆明动物研究所_动物活性蛋白多肽组学昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Graduate School of Chinese Academy of Sciences, Beijing 100049, China 3.Department of biochemistry, Kunming Medical College, Kunming, Yunnan 650032, China
推荐引用方式 GB/T 7714	Zhang Y,Yu GY,Lee WH,et al. A novel annexin A2 protein with platelet aggregation-inhibiting activity from amphibian Bombina maxima skin[J]. TOXICON,2010,56(3):458-465.
APA	Zhang Y.,Yu GY.,Lee WH.,Zhang Y[*].,Wang YJ.,...&Wei SS.(2010).A novel annexin A2 protein with platelet aggregation-inhibiting activity from amphibian Bombina maxima skin.TOXICON,56(3),458-465.
MLA	Zhang Y,et al."A novel annexin A2 protein with platelet aggregation-inhibiting activity from amphibian Bombina maxima skin".TOXICON 56.3(2010):458-465.