Molecular cloning of serine proteases from elapid snake venoms

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 动物活性蛋白多肽组学

	Molecular cloning of serine proteases from elapid snake venoms
	Jin Y; Lee WH; Zhang Y[*]
刊名	TOXICON
	2007
卷号	49 期号:8 页码:1200-1207
关键词	Serine protease Elapidae Cloning
ISSN号	0041-0101
通讯作者	zhangy@mail.kiz.ac.cn
合作状况	其它
英文摘要	Serine proteases are widely distributed in viperid snake venoms, but rare in elapid snake venoms. Previously, we have identified a fibrinogenolytic enzyme termed OhS1 from the venom of Ophiophagus hannah. The results indicated that OhS1 might be a serine protease, but there was no structural evidence previously. In the present study, the primary structure of OhS1 was determined by protein sequencing, in combination with RT-PCR and 5'-RACE methods. OhS1 precursor is composed of an 18-amino acid signal peptide, a 6-amino acid putative activation peptide and 236-amino acid mature protein. OhS1 homologues from Naja atra and Bungarus multicinctus were also cloned and reported. These elapid venom serine proteases exhibited approximately 60% sequence identity with serine proteases from the snake venoms of the Viperidae and Colubridae family. Phylogenetic analysis indicated that snake venom serine protease might have a common ancestor.
收录类别	SCI
资助信息	This work was supported by grants from Na- tional Natural Science Foundation (30630014, 30570359, 30470380 and 30670412) and the grants of ‘‘Western Light’’ Projects. Dr. Lee also received a grant from Yunnan Science and Technology Com- mission (2005PY01-23)
原文出处	2007491200.pdf
语种	英语
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/2699]
专题	昆明动物研究所_动物活性蛋白多肽组学昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	Totoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
推荐引用方式 GB/T 7714	Jin Y,Lee WH,Zhang Y[*]. Molecular cloning of serine proteases from elapid snake venoms[J]. TOXICON,2007,49(8):1200-1207.
APA	Jin Y,Lee WH,&Zhang Y[*].(2007).Molecular cloning of serine proteases from elapid snake venoms.TOXICON,49(8),1200-1207.
MLA	Jin Y,et al."Molecular cloning of serine proteases from elapid snake venoms".TOXICON 49.8(2007):1200-1207.