Purification and characterization of jerdofibrase, a serine protease from the venom of Trimeresurus jerdonii snake

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	Purification and characterization of jerdofibrase, a serine protease from the venom of Trimeresurus jerdonii snake
	Jin Y; Lu QM; Xiong YL[*]; Wei JF; Li DS; Wang WY
刊名	TOXICON
	2001
卷号	39 期号:8 页码:1203-1210
通讯作者	wangwy@mail.kiz.ac.cn
合作状况	其它
英文摘要	A fibrin(ogen)olytic serine protease from Trimeresurus jerdonii venom was identified and purified to SDS-polyacrylamide gel electrophoresis homogeneity. It is a single chain polypeptide with a molecular weight of 32 kDa under reduced condition and 28 kDa under non-reduced condition, respectively. The venom protease catalysed the hydrolysis of some chromogenic substrates such as S2238, S2160, S2302, and S2251. It degraded B beta -chain of human fibrinogen preferentially. Also the enzyme degraded fibrin directly. Its enzymatic activity was. completely inhibited by phenylmethylsulfonyl fluoride (PMSF). but not affected by EDTA. That suggested it was a serine protease. N-terminal sequence of the purified component showed high homology with other snake venom serine proteases.
收录类别	SCI
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10636]
专题	昆明动物研究所_动物毒素室昆明动物研究所_其他
作者单位	Department of Animal Toxinology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
推荐引用方式 GB/T 7714	Jin Y,Lu QM,Xiong YL[*],et al. Purification and characterization of jerdofibrase, a serine protease from the venom of Trimeresurus jerdonii snake[J]. TOXICON,2001,39(8):1203-1210.
APA	Jin Y,Lu QM,Xiong YL[*],Wei JF,Li DS,&Wang WY.(2001).Purification and characterization of jerdofibrase, a serine protease from the venom of Trimeresurus jerdonii snake.TOXICON,39(8),1203-1210.
MLA	Jin Y,et al."Purification and characterization of jerdofibrase, a serine protease from the venom of Trimeresurus jerdonii snake".TOXICON 39.8(2001):1203-1210.