A novel serine protease inhibitor from Bungarus fasciatus venom

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	A novel serine protease inhibitor from Bungarus fasciatus venom
	Lu J 1,4; Yang HL 1; Liu JZ[]2; Liang XC 1; Yu HN 2; Gao WK 2; Lai R[]1,3
刊名	PEPTIDES
	2008
卷号	23 期号:9 页码:369-374
关键词	snake venom serine protease inhibitor kunitz Bungarus fasciatus
通讯作者	rlai@mail.kiz.ac.cn ; jzliu21@heinfo.net
合作状况	其它
英文摘要	By Sephadex G-50 gel filtration, cation-exchange CM-Sephadex C-25 chromatography and reversed phase high-performance liquid chromatography (HPLC), a novel serine protease inhibitor named bungaruskunin was purified and characterized from venom of Bungarus fasciatus. Its cDNA was also cloned from the cDNA library of B. fasciatus venomous glands. The predicted precursor is composed of 83 amino acid (aa) residues including a 24-aa signal peptide and a 59-aa mature bungaruskunin. Bungaruskunin showed maximal similarity (64%) with the predicted serine protease inhibitor blackelin deduced from the cDNA sequence of the red-bellied black snake Pseudechis porphyriacus. Bungaruskunin is a Kunitz protease inhibitor with a conserved Kunitz domain and could exert inhibitory activity against trypsin, chymotrypsin, and elastase. By screening the cDNA library, two new B chains of beta-bungarotoxin are also identified. The overall structures of bungaruskunin and beta -bungarotoxin B chains are similar; especially they have highly conserved signal peptide sequences. These findings strongly suggest that snake Kunitz/BPTI protease inhibitors and neurotoxic homologs may have originated from a common ancestor.
收录类别	SCI
资助信息	This work was supported by 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Acad- emy of Sciences.
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10511]
专题	昆明动物研究所_动物毒素室昆明动物研究所_动物模型与人类重大疾病机理重点实验室昆明动物研究所_其他
作者单位	1.Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 2.College of Life Sciences School of Heibei Normal University, Shijiazhuang 050016, Hebei, China 3.Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China 4.Graduate School of the Chinese Academy of Sciences, Beijing 100009, China
推荐引用方式 GB/T 7714	Lu J,Yang HL,Liu JZ[*],et al. A novel serine protease inhibitor from Bungarus fasciatus venom[J]. PEPTIDES,2008,23(9):369-374.
APA	Lu J.,Yang HL.,Liu JZ[].,Liang XC.,Yu HN.,...&Lai R[].(2008).A novel serine protease inhibitor from Bungarus fasciatus venom.PEPTIDES,23(9),369-374.
MLA	Lu J,et al."A novel serine protease inhibitor from Bungarus fasciatus venom".PEPTIDES 23.9(2008):369-374.