The First Gene-encoded Amphibian Neurotoxin

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	The First Gene-encoded Amphibian Neurotoxin
	You DW 1,5; Hong J 2,5; Ma YF 1,5; Yang HL 1,5; Wu J 1,5; Lin DH[]2; Lai R[]1; Rong MQ 3; Yu HN 4; Liang SP 3
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2009
卷号	284 期号:33 页码:22079-22086
通讯作者	dhlin@mail.shcnc.ac.cn ; rlai@mail.kiz.ac.cn
合作状况	其它
英文摘要	Many gene-encoded neurotoxins with various functions have been discovered in fish, reptiles, and mammals. A novel 60-residue neurotoxin peptide (anntoxin) that inhibited tetrodotoxin-sensitive (TTX-S) voltage-gated sodium channel (VGSC) was purified and characterized from the skin secretions of the tree frog Hyla annectans (Jerdon). This is the first gene-encoded neurotoxin found in amphibians. The IC(50) of anntoxin for the TTX-S channel was about 3.4 mu M. Anntoxin shares sequence homology with Kunitz-type toxins but contains only two of three highly conserved cysteine bridges, which are typically found in these small, basic neurotoxin modules, i.e. snake dendrotoxins. Anntoxin showed an inhibitory ability against trypsin with an inhibitory constant (K(i)) of 0.025 mu M. Anntoxin was distributed in skin, brain, stomach, and liver with a concentration of 25, 7, 3, and 2 mu g/g wet tissue, respectively. H. annectans lives on trees or other plants for its entire life cycle, and its skin contains the largest amount of anntoxin, which possibly helps defend against various aggressors or predators. A low dose of anntoxin was found to induce lethal toxicity for several potential predators, including the insect, snake, bird, and mouse. The tissue distribution and functional properties of the current toxin may provide insights into the ecological adaptation of tree-living amphibians.
收录类别	SCI
资助信息	This work was supported in part by the Chinese National Natural Science Foundation (30830021), Chinese Academy of Sciences (KSCXZ-YW-R- 088, KSCX2-YW-G-024), and the Ministry of Science (2007AA02Z192, 2007AA100602).
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10476]
专题	昆明动物研究所_动物毒素室昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Biotoxin Units, Kunming 650223, Yunnan, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 3.Hunan Normal Univ, Coll Life Sci, Minist Educ, Key Lab Prot Chem & Dev Biol, Changsha 410081, Peoples R China 4.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Hebei, Peoples R China 5.Chinese Acad Sci, Grad Sch, Beijing 100009, Peoples R China
推荐引用方式 GB/T 7714	You DW,Hong J,Ma YF,et al. The First Gene-encoded Amphibian Neurotoxin[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2009,284(33):22079-22086.
APA	You DW.,Hong J.,Ma YF.,Yang HL.,Wu J.,...&Liang SP.(2009).The First Gene-encoded Amphibian Neurotoxin.JOURNAL OF BIOLOGICAL CHEMISTRY,284(33),22079-22086.
MLA	You DW,et al."The First Gene-encoded Amphibian Neurotoxin".JOURNAL OF BIOLOGICAL CHEMISTRY 284.33(2009):22079-22086.