Anti-thrombosis Repertoire of Blood-feeding Horsefly Salivary Glands

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 动物毒素室

	Anti-thrombosis Repertoire of Blood-feeding Horsefly Salivary Glands
	Ma DY 1,4; Wang YP 1,4; Gao L 1,4; Xu XQ 1,4; Lai R[*]1,2; Yang HL 1,4; Wu J 1,4; AN S 3,4
刊名	MOLECULAR & CELLULAR PROTEOMICS
	2009
卷号	8 期号:9 页码:2071-2079
通讯作者	rlai@mail.kiz.ac.cn
合作状况	其它
英文摘要	Blood-feeding arthropods rely heavily on the pharmacological properties of their saliva to get a blood meal and suppress immune reactions of hosts. Little information is available on antihemostatic substances in horsefly salivary glands although their saliva has been thought to contain wide range of physiologically active molecules. In traditional Eastern medicine, horseflies are used as anti-thrombosis material for hundreds of years. By proteomics coupling transcriptome analysis with pharmacological testing, several families of proteins or peptides, which exert mainly on anti-thrombosis functions, were identified and characterized from 60,000 pairs of salivary glands of the horsefly Tabanus yao Macquart (Diptera, Tabanidae). They are: (I) ten fibrin(ogen)olytic enzymes, which hydrolyze specially alpha chain of fibrin(ogen) and are the first family of fibrin(ogen) olytic enzymes purified and characterized from arthropods; (II) another fibrin(ogen)olytic enzyme, which hydrolyzes both alpha and beta chain of fibrin(ogen); (III) ten Arg-Gly-Asp-motif containing proteins acting as platelet aggregation inhibitors; (IV) five thrombin inhibitor peptides; (V) three vasodilator peptides; (VI) one apyrase acting as platelet aggregation inhibitor; (VII) one peroxidase with both platelet aggregation inhibitory and vasodilator activities. The first three families are belonging to antigen five proteins, which show obvious similarity with insect allergens. They are the first members of the antigen 5 family found in salivary glands of blood sucking arthropods to have anti-thromobosis function. The current results imply a possible evolution from allergens of blood-sucking insects to anti-thrombosis agents. The extreme diversity of horsefly anti-thrombosis components also reveals the anti-thrombosis molecular mechanisms of the traditional Eastern medicine insect material. Molecular & Cellular Proteomics 8: 2071-2079, 2009.
收录类别	SCI
资助信息	This work was supported by the Chinese National Natural Sci- ence Foundation (30830021), Chinese Academy of Sciences (KSCXZ-YW-R-088, KSCX2-YW-G-024), and the Ministry of Sci- ence (2008AA02Z133, 2007AA100602).
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10474]
专题	昆明动物研究所_动物毒素室昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 2.Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China 3.School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230026, China 4.Graduate School of the Chinese Academy of Sciences, Beijing 100009, China
推荐引用方式 GB/T 7714	Ma DY,Wang YP,Gao L,et al. Anti-thrombosis Repertoire of Blood-feeding Horsefly Salivary Glands[J]. MOLECULAR & CELLULAR PROTEOMICS,2009,8(9):2071-2079.
APA	Ma DY.,Wang YP.,Gao L.,Xu XQ.,Lai R[*].,...&AN S.(2009).Anti-thrombosis Repertoire of Blood-feeding Horsefly Salivary Glands.MOLECULAR & CELLULAR PROTEOMICS,8(9),2071-2079.
MLA	Ma DY,et al."Anti-thrombosis Repertoire of Blood-feeding Horsefly Salivary Glands".MOLECULAR & CELLULAR PROTEOMICS 8.9(2009):2071-2079.