Expression and characterization of a fibrinogenolytic enzyme from horsefly salivary gland.

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 动物毒素室

	Expression and characterization of a fibrinogenolytic enzyme from horsefly salivary gland.
	Sheng X 1; Cao L 2; Meng P 2,4,5; Chen WL[]6; Lu QM[]2,4,5; Lu XC 2,3; Wang YF 2,3; Han YL 2,3
刊名	PROTEIN EXPRESSION AND PURIFICATION
	2017
卷号	129 期号:X 页码:135-142
关键词	Anticoagulant Fibrinogenolytic enzyme Horsefly SCP domain Salivary gland
通讯作者	chenwenlin@aliyun.com
合作状况	其它
英文摘要	Enzymes from various natural resources are valuable in management of thrombosis. Blood-sucking arthropods are one of these resources because they have a wide array of anti-hemostasis molecules in their salivary gland. However, it is difficult to purify enough protein samples from the salivary glands for pharmacological studies. In this work, a fibrinogenolytic enzyme (tablysin 2) identified from salivary glands of the horsefly Tabanus yao was expressed in Escherichia coli to further study its biological activities. The primary structure of tablysin 2 showed significant domain similarity to arthropod proteins from the antigen 5 family containing SCP domain, whose biological functions are poorly understood. Tablysin 2 cleaved the Aα and part of Bβ chains of fibrinogen and did not affect γ chain and fibrin. It inhibited platelet aggregation induced by ADP. It did not directly induce hemorrhage or activate plasminogen. The fibrinogenolytic activity of tablysin 2 provides a clue for the functions of antigen 5-related proteins in other haematophagous arthropods. This work demonstrate a method of expression of arthropod salivary proteins which are difficult to obtain from natural resources for further functional studies.
收录类别	SCI
资助信息	This work was supported by the Ministry of Science and Technology (2013CB911300, 2010CB529800), Chinese National Natural Science Foundation (31025025, 31260208, U1132601, 31200590), Chinese Academy of Sciences (SAJC201308, Y302B01021) and Yunnan Province (P0120150010, 2015BC005).
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/9773]
专题	昆明动物研究所_动物毒素室昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.The Affiliated Stomatological Hospital of Kunming Medicinal College, Kunming, 650221, Yunnan, China 2.Key Laboratory of Animal Models and Human Disease Mechanism of the Chinese Academy of Science & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Science, Kunming, Yunnan, 650223, China 3.Graduate University of Chinese Academy of Sciences, Beijing, 100009, China 4.United Laboratory of Natural Peptide of University of Science and Technology of China, Kunming Institute of Zoology, Chinese Academy of Science, Kunming, Yunnan, 650223, China 5.Sino-African Joint Research Center, Chinese Academy of Science, Wuhan, Hubei, 430074, China 6.Yunnan Clinical Research Center of Breast Cancer, The Third Affiliated Hospital of Kunming Medical College, Kunming, 650032, China
推荐引用方式 GB/T 7714	Sheng X,Cao L,Meng P,et al. Expression and characterization of a fibrinogenolytic enzyme from horsefly salivary gland.[J]. PROTEIN EXPRESSION AND PURIFICATION,2017,129(X):135-142.
APA	Sheng X.,Cao L.,Meng P.,Chen WL[].,Lu QM[].,...&Han YL.(2017).Expression and characterization of a fibrinogenolytic enzyme from horsefly salivary gland..PROTEIN EXPRESSION AND PURIFICATION,129(X),135-142.
MLA	Sheng X,et al."Expression and characterization of a fibrinogenolytic enzyme from horsefly salivary gland.".PROTEIN EXPRESSION AND PURIFICATION 129.X(2017):135-142.