A novel myotoxin from the venom of Trimeresurus mucrosquamatus

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	A novel myotoxin from the venom of Trimeresurus mucrosquamatus
	Xu TR; Wang WY; Meng QX; Huang YH; Lu QM; Xiong YL[*]
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	1999
卷号	31 期号:5 页码:483-488
关键词	myotoxin phospholipase A(2) platelet Trimeresurus mucrosquamatus venom
其他题名	一种新的烙铁头蛇毒肌肉毒素
通讯作者	xutr@163.net
合作状况	其它
中文摘要	用分子筛和快速蛋白质液相色谱(FPLC)从烙铁头(Trimeresurus mucrosquamatus)蛇毒中分离了一个新的碱性肌肉毒素, 命名为 TMPB。它的分子量为 16 000, 等电点为 9.2。用蛋白质序列仪测定了其 N 端 24 个氨基酸残基, TMPB 与其他两个从同种蛇毒中分离到的碱性磷酯酶 A 2 的同源性分别为 41.7%和 54.2%。 TMPB 没有明显的磷酯酶 A 2 水解活性, 但它的肌肉毒性和血小板聚集抑制活性却极强, 其肌肉毒性和血小板聚集抑制活性可被肝素所抑制。
英文摘要	A novel myotoxin , designated TMPB , was purified from the venom of Trimeresurus mucrosquamatus by Sephadex G-100 superfine gel chromatography and fast protein liquid chromatography (FPLC).The N-terminal sequence of 24 amino acid residues was determined by protein sequencer.The sequence similarities between TMPB and other two phospholipase A 2 (PLA 2 s)previously purified from the same venom were 41.7 %and 54 .2%, respectively , but TMPB showed no detectable PLA 2 hydrolytic activity .Its molecular weight was estimated to be 16 000 by reducing SDS-PAGE and isoelectric point wasdetermined to be 9.2 by isoelectric focusing electrophoresis.TMPB exhibited strong myotoxicity and platelet aggregation inhibiting activity , and the two activities could all be inhibited by heparin.
收录类别	其他
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/9679]
专题	昆明动物研究所_动物毒素室昆明动物研究所_其他
作者单位	Kunming Institute of Zoology , the Chinese Academy of Sciences, K unming 650223, China
推荐引用方式 GB/T 7714	Xu TR,Wang WY,Meng QX,et al. A novel myotoxin from the venom of Trimeresurus mucrosquamatus[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,1999,31(5):483-488.
APA	Xu TR,Wang WY,Meng QX,Huang YH,Lu QM,&Xiong YL[*].(1999).A novel myotoxin from the venom of Trimeresurus mucrosquamatus.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,31(5),483-488.
MLA	Xu TR,et al."A novel myotoxin from the venom of Trimeresurus mucrosquamatus".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 31.5(1999):483-488.