A Novel Trypsin Inhibitor-Like Cysteine-Rich Peptide from the Frog Lepidobatrachus laevis Containing Proteinase-Inhibiting Activity

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	A Novel Trypsin Inhibitor-Like Cysteine-Rich Peptide from the Frog Lepidobatrachus laevis Containing Proteinase-Inhibiting Activity
	Wang YW 1; Tan JM 1; Lai R 1,2; Lu QM[*]2; Du CW 1; Luan N 1; Yan XW 1
刊名	Natural Products and Bioprospecting
	2015
卷号	5 期号:4 页码:209-214
关键词	Amphibian Cysteine-rich peptide Skin Trypsin inhibitor
通讯作者	lvqm@aliyun.com.
合作状况	其它
英文摘要	Various bio-active substances in amphibian skins play important roles in survival of the amphibians. Many protease inhibitor peptides have been identified from amphibian skins, which are supposed to negatively modulate the activity of proteases to avoid premature degradation or release of skin peptides, or to inhibit extracellular proteases produced by invading bacteria. However, there is no information on the proteinase inhibitors from the frog Lepidobatrachus laevis which is unique in South America. In this work, a cDNA encoding a novel trypsin inhibitor-like (TIL) cysteine-rich peptide was identified from the skin cDNA library of L. laevis. The 240-bp coding region encodes an 80-amino acid residue precursor protein containing 10 half-cysteines. By sequence comparison and signal peptide prediction, the precursor was predicted to release a 55-amino acid mature peptide with amino acid sequence, IRCPKDKIYKFCGSPCPPSCKDLTPNCIAVCKKGCFCRDGTVDNNHGKCVKKENC. The mature peptide was named LL-TIL. LL-TIL shares significant domain similarity with the peptides from the TIL supper family. Antimicrobial and trypsin-inhibitory abilities of recombinant LL-TIL were tested. Recombinant LL-TIL showed no antimicrobial activity, while it had trypsin-inhibiting activity with a Ki of 16.5178 μM. These results suggested there was TIL peptide with proteinase-inhibiting activity in the skin of frog L. laevis. To the best of our knowledge, this is the first report of TIL peptide from frog skin.
收录类别	SCI
资助信息	This work was supported by grants from the China National Natural Science Foundation of Young Scientists (No. 31201717), Special Foundation for Young Scientists of Jiangsu Pro- vince (No. BK2012365), Jiangsu Province Science and Technology Support Project (No. BE2012748).
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/9448]
专题	昆明动物研究所_动物毒素室昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China 2.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China
推荐引用方式 GB/T 7714	Wang YW,Tan JM,Lai R,et al. A Novel Trypsin Inhibitor-Like Cysteine-Rich Peptide from the Frog Lepidobatrachus laevis Containing Proteinase-Inhibiting Activity[J]. Natural Products and Bioprospecting,2015,5(4):209-214.
APA	Wang YW.,Tan JM.,Lai R.,Lu QM[*].,Du CW.,...&Yan XW.(2015).A Novel Trypsin Inhibitor-Like Cysteine-Rich Peptide from the Frog Lepidobatrachus laevis Containing Proteinase-Inhibiting Activity.Natural Products and Bioprospecting,5(4),209-214.
MLA	Wang YW,et al."A Novel Trypsin Inhibitor-Like Cysteine-Rich Peptide from the Frog Lepidobatrachus laevis Containing Proteinase-Inhibiting Activity".Natural Products and Bioprospecting 5.4(2015):209-214.