Vascular endothelial growth factor from Trimeresurus jerdonii venom specifically binds to VEGFR-2

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	Vascular endothelial growth factor from Trimeresurus jerdonii venom specifically binds to VEGFR-2
	Zhong SR 1; Wu JB 2; Cui YP 3; Li R 2; Zhu SW 2; Rong MQ 2,4; Lu QM[]2,4; Lai R[]2,4
刊名	BIOCHIMIE
	2015
卷号	116 期号:X 页码:1-7
关键词	Molecular cloning Purification Trimeresurus jerdonii VEGFR Vascular endothelial growth factor
通讯作者	lvqm@mail.kiz.ac.cn ; rlai@mail.kiz.ac.cn
合作状况	其它
英文摘要	Vascular endothelial growth factors (VEGFs) play important roles in angiogenesis. In this study, a vascular endothelial growth factor named TjsvVEGF was purified from the venom of Trimeresurus jerdonii by gel filtration, affinity, ion-exchange and high-performance liquid chromatography. TjsvVEGF was a homodimer with an apparent molecular mass of 29 kDa. The cDNA encoding TjsvVEGF was obtained by PCR. The open reading frame of the cloned TjsvVEGF was composed of 432 bp coding for a signal peptide of 24 amino acid residues and a mature protein of 119 amino acid residues. Compared with other snake venom VEGFs, the nucleotide and deduced protein sequences of the cloned TjsvVEGF were conserved. TjsvVEGF showed low heparin binding activity and strong capillary permeability increasing activity. The KD of TjsvVEGF to VEFGR-2 is 413 pM. However, the binding of TjsvVEGF to VEGFR-1 is too weak to detect. Though TjsvVEGF had high sequence identities (about 90%) with Crotalinae VEGFs, the receptor preference of TjsvVEGF was similar to Viperinae VEGFs which had lower sequence identities (about 60%) with it. TjsvVEGF might serve as a useful tool for the study of structure-function relationships of VEGFs and their receptors.
收录类别	SCI
资助信息	This work was supported by the grant from Chinese National Natural Science Foundation (31372208, 31260208, U1302221, U1132601), the Ministryof Science and Technology (2013CB911304, 2011ZX09102-002-10, 2014ZX0800949B), and CAS (Y302B01021).
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/9374]
专题	昆明动物研究所_动物毒素室昆明动物研究所_动物模型与人类重大疾病机理重点实验室昆明动物研究所_其他
作者单位	1.Department of Forensic Biology, School of Forensic Medicine, Kunming Medical University, Kunming, Yunnan 650500, China 2.Key Laboratory of Animal Models and Human Disease Mechanism of the Chinese Academy of Science & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Science, Kunming, Yunnan 650223, China 3.Department of Biochemistry, Cangzhou Medical College, Cangzhou, Hebei 061001, China 4.Jointed Laboratory of Natural Bioactive Peptide of Kunming Institute of Zoology, Chinese Academy of Science & University of Science and Technology of China, Kunming Institute of Zoology, Chinese Academy of Science, Kunming, Yunnan 650223, China
推荐引用方式 GB/T 7714	Zhong SR,Wu JB,Cui YP,et al. Vascular endothelial growth factor from Trimeresurus jerdonii venom specifically binds to VEGFR-2[J]. BIOCHIMIE,2015,116(X):1-7.
APA	Zhong SR.,Wu JB.,Cui YP.,Li R.,Zhu SW.,...&Lai R[*].(2015).Vascular endothelial growth factor from Trimeresurus jerdonii venom specifically binds to VEGFR-2.BIOCHIMIE,116(X),1-7.
MLA	Zhong SR,et al."Vascular endothelial growth factor from Trimeresurus jerdonii venom specifically binds to VEGFR-2".BIOCHIMIE 116.X(2015):1-7.