Five novel antimicrobial peptides from the Kuhl's wart frog skin secretions, Limnonectes kuhlii

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	Five novel antimicrobial peptides from the Kuhl's wart frog skin secretions, Limnonectes kuhlii
	Wang GX 1; Wang Y 2,3; Ma DY 2; Liu H 2; Li JX 2; Zhang KY 1; Yang XL 4; Lai R[]1,2; Liu JZ[]4
刊名	MOLECULAR BIOLOGY REPORTS
	2013
卷号	40 期号:2 页码:1097-1102
关键词	Antimicrobial peptides Amphibian Skin Limnonectes kuhlii
通讯作者	rlai72@njau.edu.cn ; jzliu21@heinfo.net
合作状况	其它
英文摘要	Five novel antimicrobial peptides (temporin-LK1, rugosin-LK1, rugosin-LK2, gaegurin-LK1, and gaegurin-LK2) are purified and characterized from Kuhl's wart frog skin secretions, Limnonectes kuhlii. They share obvious similarity to temporin, rugosin, and gaegurin antimicrobial peptide family, respectively. Their amino acid sequences were determined by Edman degradation and mass spectrometry, and further confirmed by cDNA cloning. Nine cDNA sequences encoding precursors of these five purified antimicrobial peptides and other four hypothetical antimicrobial peptides were cloned from the skin cDNA library of L. kuhlii. The deduced precursors are composed of a predicted signal peptide, an acidic spacer peptide, and a mature antimicrobial peptide. Most of them showed strong antimicrobial activities against Gram-positive and Gram-negative bacteria and fungi. The current work identified and characterized three families of antimicrobial peptides from L. kuhlii skins and confirmed that the genus of Limnonectes amphibians share similar antimicrobial peptide families with the genus of Rana amphibians. In addition, a unique antimicrobial peptide (temporin-LK1) with 17 residues including four phenylalanines, which is significantly different from other temporins (16 residues, one or two phenylalanines), was identified in this work. Such unique structure might provide novel template or leading structure to design antimicrobial agents.
收录类别	SCI
资助信息	This work was supported by Chinese Academy of Sciences (KSCX2-YW-G-024), the Ministry of Science and Technol- ogy (2010CB529800), the Ministry of Agriculture (2009ZX08009- 159B), and Health Bureau of Shanghai (09411952800).
语种	英语
WOS记录号	WOS:000313165500040
公开日期	2014-10-23
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/8133]
专题	昆明动物研究所_动物毒素室昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China 2.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100009, China 4.College of Life Sciences, Hebei Normal University, Shijiazhuang 050016, Hebei, China
推荐引用方式 GB/T 7714	Wang GX,Wang Y,Ma DY,et al. Five novel antimicrobial peptides from the Kuhl's wart frog skin secretions, Limnonectes kuhlii[J]. MOLECULAR BIOLOGY REPORTS,2013,40(2):1097-1102.
APA	Wang GX.,Wang Y.,Ma DY.,Liu H.,Li JX.,...&Liu JZ[*].(2013).Five novel antimicrobial peptides from the Kuhl's wart frog skin secretions, Limnonectes kuhlii.MOLECULAR BIOLOGY REPORTS,40(2),1097-1102.
MLA	Wang GX,et al."Five novel antimicrobial peptides from the Kuhl's wart frog skin secretions, Limnonectes kuhlii".MOLECULAR BIOLOGY REPORTS 40.2(2013):1097-1102.