Alpha-Actinin Is a New Type of House Dust Mite Allergen

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	Alpha-Actinin Is a New Type of House Dust Mite Allergen
	An S 1,4; Shen CB 1,4; Liu XY 2; Chen LL 3; Xu XM 3; Rong MQ[]1; Liu ZG[]2; Lai R[*]1
刊名	PLOS ONE
	2013
卷号	8 期号:12 页码:e81377
通讯作者	mingqiangrong@mail.kiz.ac.cn ; lzg195910@126.com ; rlai@mail.kiz.ac.cn
合作状况	其它
英文摘要	Main indoor allergens for humans are from house dust mites. There are more than 30 allergens in Dermatophagoides farinae but only fourteen allergens have been identified from this mite including Der f 1-3, 6, 7, 10, 11, 13-18, and 22. A native allergen protein (Der f 24, 90 kDa) was purified from D. farinae by gel filtration and anionic exchange liquid chromatography combined with IgE immunodetection. Its primary structure was determined by Edman degradation, mass spectrometry analysis and cDNA cloning. Enzyme-linked immunosorbent assay inhibition tests (ELISA-IT), immunoblots, basophil activation test (BAT) and skin prick test (SPT) were performed to evaluate the allergenicity. It was identified as an alpha (alpha)-actinin containing a CaM-like domain with EF-hand motifs. Der f 24 reacted to sera from 85.4% (35/41) of patients on western blot analysis. It reduced similar to 20% sera IgE reactivity to D. farinae extracts on a competitive ELISA. Eighty percent (8/10) of patients with D. farinae allergy showed positive reactions to Der f 24 in skin prick test. The expression of CD63 on basophils from patients was up-regulated by Der f 24 by similar to 5.4-fold. Alpha-actinin was identified as a new type of house dust mite allergen. To the best of our knowledge, this is the first report of alpha-actinin as an allergen.
收录类别	SCI
资助信息	This work was supported by the Ministry of Science and Technology (2010CB529800,2013CB911300) and Chinese National Natural Science Foundation (31070701, 31000962, 31025025, 30971179, 30972848, 31028006).
语种	英语
WOS记录号	WOS:000328566700035
公开日期	2014-09-26
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/8012]
专题	昆明动物研究所_动物毒素室昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China 2.Institute of Allergy and Immunology, Shenzhen University, Shenzhen, China 3.Clinical Laboratory, The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan, China 4.Graduate School of the Chinese Academy of Sciences, Beijing, China
推荐引用方式 GB/T 7714	An S,Shen CB,Liu XY,et al. Alpha-Actinin Is a New Type of House Dust Mite Allergen[J]. PLOS ONE,2013,8(12):e81377.
APA	An S.,Shen CB.,Liu XY.,Chen LL.,Xu XM.,...&Lai R[*].(2013).Alpha-Actinin Is a New Type of House Dust Mite Allergen.PLOS ONE,8(12),e81377.
MLA	An S,et al."Alpha-Actinin Is a New Type of House Dust Mite Allergen".PLOS ONE 8.12(2013):e81377.