Different Expression Profiles of Bioactive Peptides in Pelophylax nigromaculatus from Distinct Regions

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	Different Expression Profiles of Bioactive Peptides in Pelophylax nigromaculatus from Distinct Regions
	Song YZ[]1; Ji SL 1; Liu W 1; Yu XY 1; Meng QX 1; Lai R[]2,3
刊名	BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
	2013
卷号	77 期号:5 页码:1075-1079
关键词	amphibian skin antimicrobial peptides antioxidant peptide different expression cDNA screening
通讯作者	uzhusong@kmust.edu.cn ; rlai@mail.kiz.ac.cn
合作状况	其它
英文摘要	Amphibian skin is an abundant repository of bioactive peptides, important components of the defensive system. The variability of the bioactive peptide repertoires of individual species remains unclear. In this study, dark-spotted frogs were collected from Kunming in Yunnan Province, China and Guiyang in Guizhou Province, China to determine whether the bioactive peptides in amphibian skin differ between the two regions. Eight-antimicrobial peptides and an antioxidant peptide were identified by screening of cDNA library. Among the identified peptides, three antimicrobial peptides (pelophylaxin-2GY, temporin-1GY, and temporin-1KM) and an antioxidant peptide (antioxidin-PN) are reported here for the first time. Nigrocin-1, nigrocin-2, and pelophylaxin-2 were expressed by frogs in both regions. Pelophylaxin-2GY and temporin-1GY were found only in the frogs from Guiyang, whereas antioxidin-PN, esculetin-1, esculetin-2, and temporin-1KM were found only in those from Kunming. This difference was confirmed by allele-specific RT-PCR. The bioactive peptides expressed clearly varied between these populations of the same species.
收录类别	SCI
语种	英语
WOS记录号	WOS:000320414900029
公开日期	2013-08-08
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7581]
专题	昆明动物研究所_动物毒素室昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Engineering Research Center for Molecular Diagnosis, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnam, China 2.Chinese Acad Sci, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Biotoxin Units, Kunming 650223, Yunnan, Peoples R China 3.Nanjing Agr Univ, Life Sci Coll, Nanjing 210095, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Song YZ[*],Ji SL,Liu W,et al. Different Expression Profiles of Bioactive Peptides in Pelophylax nigromaculatus from Distinct Regions[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2013,77(5):1075-1079.
APA	Song YZ[],Ji SL,Liu W,Yu XY,Meng QX,&Lai R[].(2013).Different Expression Profiles of Bioactive Peptides in Pelophylax nigromaculatus from Distinct Regions.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,77(5),1075-1079.
MLA	Song YZ[*],et al."Different Expression Profiles of Bioactive Peptides in Pelophylax nigromaculatus from Distinct Regions".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 77.5(2013):1075-1079.