A novel myotropic peptide from the skin secretions of the tree frog, Polypedates pingbianensis | |
Yan HL1; Wei L1; He XQ1; Liu H2; Yang SL2; Lai R[*]1,2; Rao DQ[*]2 | |
刊名 | BIOCHIMIE |
2012 | |
卷号 | 94期号:8页码:1718-1723 |
关键词 | Amphibian Skin Defensive Myotropic Polypedates pingbianensis |
通讯作者 | rlai72@njau.edu.cn ; raodq@mail.kiz.ac.cn |
合作状况 | 其它 |
英文摘要 | A novel myotropic peptide, polypedatein, was purified and characterized from the skin secretions of the tree frog, Polypedates pingbianensis. Its primary structure, TLLCKYFAIC, was determined by Edman degradation and mass spectrometry. Polypedatein was subjected to bioassays including myotropic, antimicrobial, and serine protease inhibitory activities, which are related with many amphibian skin bioactive peptides. It was found to elicit concentration-dependent contractile effects on isolated rat ileum. cDNA clones encoding the precursor of polypedatein were isolated by screening a skin cDNA library of P. pingbianensis and then sequenced. The amino acid sequence deduced from the cDNA sequences matches well with the result from Edman degradation. BLAST search revealed that the sequence of polypedatein did not show similarity to known protein or peptide sequences. Especially, polypedatein does not contain conserved structural motifs of other amphibian myotropic peptides, such as bradykinins, bombesins, cholecystokinin (CCK), and tachykinins, indicating that polypedatein belongs to a novel amphibian myotropic peptide family. The signal peptide of the precursor encoding polypedatein shows significant sequence identity to that of other amphibian skin defensive peptides, such as antimicrobial peptides, bradykinins, lectins, and serine protease inhibitors, suggesting that polypedatein belongs to a novel amphibian myotropic peptide family. Polypedatein is also the first bioactive peptide from the genus of the frog, Polypedates. |
收录类别 | SCI |
资助信息 | This work was supported by Chinese National Natural Science Foundation (30830021, 31025025, 31070701, 31000960, 31025025, U1132601),theMinistryofScienceandTechnology(2010CB529800, 2009ZX09103-1/091, 2011ZX09102-002-10), the Ministry of Agri- culture (2009ZX08009-159B) and Yunnan Province (Y103951111). |
语种 | 英语 |
WOS记录号 | WOS:000306209000010 |
公开日期 | 2012-09-13 |
内容类型 | 期刊论文 |
源URL | [http://159.226.149.42:8088/handle/152453/7068] |
专题 | 昆明动物研究所_兽类生态与进化 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_动物毒素室 |
作者单位 | 1.Life Sciences College of Nanjing Agricultural University, 1st Weigang, Nanjing, Jiangsu 210095, China 2.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Provinces, Kunming Institute of Zoology, Kunming 650223, Yunnan, China |
推荐引用方式 GB/T 7714 | Yan HL,Wei L,He XQ,et al. A novel myotropic peptide from the skin secretions of the tree frog, Polypedates pingbianensis[J]. BIOCHIMIE,2012,94(8):1718-1723. |
APA | Yan HL.,Wei L.,He XQ.,Liu H.,Yang SL.,...&Rao DQ[*].(2012).A novel myotropic peptide from the skin secretions of the tree frog, Polypedates pingbianensis.BIOCHIMIE,94(8),1718-1723. |
MLA | Yan HL,et al."A novel myotropic peptide from the skin secretions of the tree frog, Polypedates pingbianensis".BIOCHIMIE 94.8(2012):1718-1723. |
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