Gene cloning and characterization of novel antinociceptive peptide from the brain of the frog, Odorrana grahami

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	Gene cloning and characterization of novel antinociceptive peptide from the brain of the frog, Odorrana grahami
	Chen WL 1,4; Yang XN 3; Chen LL 1,5; Yang XL 1; Feng FF 1; He WY 1; Yu HN[]1,2; Liu JZ[]1
刊名	BIOCHIMIE
	2011
卷号	93 期号:7 页码:1110-1114
关键词	Amphibian Nociceptive Opiate peptide Odorrana grahami Defense
通讯作者	jzliu21@heinfo.net ; joannyu@live.cn
合作状况	其它
英文摘要	Amphibian opiate peptides including dermorphins and deltorpins have been recently found only in the skin of South American frogs belonging to the subfamily Phyllomedusinae (Phyllomedusa, Agalychnis and Pachymedusa species). No opiate peptides have ever been identified from other amphibians or organs except skin. Here we report the purification and characterization of a novel antinociceptive peptide named odorranaopin from the homogenates of the frog brains, Odorrana grahami, which is also the first antinociceptive peptide found in Ranidae amphibian. Odorranaopin comprises 17 amino acid residues with the sequence of DYTIRTRLHQESSRKVL (Mr 2102 Da). The cDNA encoding odorranaopin was cloned from the frog brain cDNA library, and it was confirmed to be a specific gene. The odorranaopin precursor deduced is composed of 61 amino acid residues including the predicted signal peptide, acidic spacer peptide and mature odorranaopin positioned at the C-terminus. Odorranaopin could inhibit nociceptive responses induced by formalin and acetic acid. It also inhibited the contractile responses of ileum smooth muscle induced by bradykinin, implying that the antinociceptive activity of odorranaopin possibly results from its blockade on bradykinin or bradykinin receptor functions. Odorranaopin is the first antinociceptive peptide found in Ranidae amphibian.
收录类别	SCI
资助信息	This work was supported by the grants from Chinese National Natural Science Foundation (30900240, 41076098).
语种	英语
公开日期	2011-08-09
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/353002/6637]
专题	昆明动物研究所_动物毒素室昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050016, China 2.School of Life Science and Biotechnology, Dalian University of Technology, Dalian, Liaoning 116024, China 3.Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 4.Yunnan Clinical Research Center of Breast Cancer, The Third Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan, China 5.Clinical Laboratory, The First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan, China
推荐引用方式 GB/T 7714	Chen WL,Yang XN,Chen LL,et al. Gene cloning and characterization of novel antinociceptive peptide from the brain of the frog, Odorrana grahami[J]. BIOCHIMIE,2011,93(7):1110-1114.
APA	Chen WL.,Yang XN.,Chen LL.,Yang XL.,Feng FF.,...&Liu JZ[*].(2011).Gene cloning and characterization of novel antinociceptive peptide from the brain of the frog, Odorrana grahami.BIOCHIMIE,93(7),1110-1114.
MLA	Chen WL,et al."Gene cloning and characterization of novel antinociceptive peptide from the brain of the frog, Odorrana grahami".BIOCHIMIE 93.7(2011):1110-1114.