Purif ication and Characterization of Jerdonitin ,a Non2hemorrhagic Metalloproteinase fromTrimeresurus jerdonii Venom

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	Purif ication and Characterization of Jerdonitin ,a Non2hemorrhagic Metalloproteinase fromTrimeresurus jerdonii Venom
	Chen RQ 1,2; Jin Y 1; Wu JB 1,2; Zhong SR 1,2; Zhu SW 1; Lu QM[1]1; Wang WY 1; Xiong YL[*]1
刊名	动物学研究
	2005
卷号	26 期号:6 页码:616-621
关键词	Trimeresurus jerdonii SVMP Hemorrhage Fibrinogenolytic activity
其他题名	菜花烙铁头蛇毒金属蛋白酶Jerdonitin的分离纯化和理化性质
通讯作者	xiongyl@mail.kiz.ac.cn
合作状况	其它
中文摘要	以前从菜花烙铁头蛇毒中分离纯化到Jerdonitin。与其他Ⅱ型蛇毒金属蛋白酶相比,Jerdonitin由金属蛋白酶和去整合素两个结构域组成。但没有检测到其出血和纤维蛋白原降解活性,推测可能高压液相色谱的有机溶液影响了其酶活性。采用不含高压液相色谱柱层析的新分离手段分离得到Jerdonitin。Jerdonitin在还原和非还原SDS-PAGE电泳中分别呈现一条表观分子量为38和36kDa的条带。像其他典型的蛇毒金属蛋白酶一样,Jerdonitin优先降解人纤维蛋白原的alpha链,并且该活性能被EDTA完全抑制,而PMSF对其没有影响。Jer-donitin不诱导小白鼠皮下出血。
英文摘要	Previously, we have purified Jerdonitin from Trimeresurusjerdonii venom. Compared with other P-Ⅱ class snake venom metalloproteinases （SVMPs）, Jerdonitin has a primary structure comprising metalloproteinase and disintegrin domains. However, no hemorrhagic and fibrinogenolytic activities were detected for Jerdonitin. We thought that organic buffer of high performance liquid charamatography （HPLC） might affect its enzymatic activity. In this study, we purified Jerdonitin by another procedure excluding the HPLC. It was homogenous as judged by SDS-PAGE and had an apparent molecular weight of 36 kDa under non-reducing conditions and 38 kDa under reducing conditions, respectively. Like other typical metalloproteinases, Jerdonitin preferentially degraded alpha-chain of human fibrinogen and this fibrinogenolytic activity was completely inhibited by EDTA, but not by PMSF. It was interesting that Jerdonitin did not induce hemorrhage after intradermal injection in mice.
收录类别	其他
资助信息	This work was supported by Natural Science Foundation of Yunnan Science and Technology Committee (2002C0063M)
语种	英语
公开日期	2010-08-25
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/6043]
专题	昆明动物研究所_动物毒素室昆明动物研究所_其他
作者单位	1.Department of Animal Toxinology, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	Chen RQ,Jin Y,Wu JB,et al. Purif ication and Characterization of Jerdonitin ,a Non2hemorrhagic Metalloproteinase fromTrimeresurus jerdonii Venom[J]. 动物学研究,2005,26(6):616-621.
APA	Chen RQ.,Jin Y.,Wu JB.,Zhong SR.,Zhu SW.,...&Xiong YL[*].(2005).Purif ication and Characterization of Jerdonitin ,a Non2hemorrhagic Metalloproteinase fromTrimeresurus jerdonii Venom.动物学研究,26(6),616-621.
MLA	Chen RQ,et al."Purif ication and Characterization of Jerdonitin ,a Non2hemorrhagic Metalloproteinase fromTrimeresurus jerdonii Venom".动物学研究 26.6(2005):616-621.