Purification, characterization and biological activity of an L-amino acid oxidase from Trimeresurus mucrosquamatus venom | |
Wei JF1,2; Wei Q1; Lu QM1; Tai H; Jin Y1; Wang WY1; Xiong YL[*]1 | |
刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
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2003 | |
卷号 | 35期号:3页码:219-224 |
关键词 | L-amino acid oxidase Trimeresurus mucrosquamatus antibacterial activity cytotoxicity platelet aggregation |
ISSN号 | 0582-9879 |
其他题名 | 烙铁头蛇毒L-2氨基酸氧化酶的分离、纯化及其生物学活性 |
通讯作者 | wangwy@mail. kiz.ac.cn |
合作状况 | 其它 |
中文摘要 | 通过 DEAE Sephadex A 2 50 阴离子交换柱 , Sephadex G 2 75 分子筛 , Resourse Q 阴离子交换柱三步层析从湖南产的烙铁头蛇毒中分离、 纯化得到一个 L 2氨基酸氧化酶(TM2 LAO) , 它由两个非共价的亚基组成 , 每个亚基的分子量为 55 kD 。 与台湾产的烙铁头蛇毒 L 2氨基酸氧化酶分子量 (70 kD) 不同。 TM 2 LAO 的 N 末端氨基酸序列是 ADNK NPLEECFRETNYEEFLEIAR , 与报 道的蝰科的 L 2氨基酸氧化酶的相似性比眼镜蛇科的要高。 TM 2 LAO 能抑制大肠杆菌、 金黄色葡萄球菌和痢疾杆菌的生长 , 杀死肿瘤细胞以及诱导血小板聚集。 这些活性能被过氧化氢酶所抑制 , 说明 TM 2 LAO 生理学功能主要是通过酶反应产生的过氧化氢(H2 O 2 ) 介导的。 |
英文摘要 | An L-amino acid oxidase (TM-LAO) from the venom of Hunan Trimeresurus mucrosquamatus was purified to homogeneity by three steps including DEAE Sephadex A-50 ion-exchange chromatography, Sephadex G-75 gel filtration and Resource Q ion-exchange chromatography. TM-LAO is composed of two identical subunits with a molecular weight of 55 kD by SDS-polyacrylamide gel electrophoresis. The molecular weight was different with that of LAO purified from the same species distributed in Taiwan that was 70 kD. The 24 N-terminal amino acid sequence of TM-LAO is ADNKNPLEECFRETNYEEFLEIAR, which shares high similarity with other Viperid snake venom LAOs and has moderate similarity with Elapid snake venom LAOs. Further studies found that TM-LAO inhibited the growth of E. coli, S. aurues and B. dysenteriae. TM-LAO also showed cytotoxicity and platelet aggregation activity. All the biological activities were eliminated by catalase, a H(2)O(2) scavenger. It was shown that these biological effects were possibly due to the formation of H(2)O(2) produced by TM-LAO. |
收录类别 | SCI |
原文出处 | 200335219.pdf |
语种 | 英语 |
公开日期 | 2010-08-24 |
内容类型 | 期刊论文 |
源URL | [http://159.226.149.42:8088/handle/152453/5283] ![]() |
专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_其他 |
作者单位 | 1.Chinese Acad Sci, Kunming Inst Zool, Dept Anim Toxicol, Kunming 650223, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Wei JF,Wei Q,Lu QM,et al. Purification, characterization and biological activity of an L-amino acid oxidase from Trimeresurus mucrosquamatus venom[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(3):219-224. |
APA | Wei JF.,Wei Q.,Lu QM.,Tai H.,Jin Y.,...&Xiong YL[*].(2003).Purification, characterization and biological activity of an L-amino acid oxidase from Trimeresurus mucrosquamatus venom.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(3),219-224. |
MLA | Wei JF,et al."Purification, characterization and biological activity of an L-amino acid oxidase from Trimeresurus mucrosquamatus venom".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.3(2003):219-224. |
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