Comparative Study of the Biological Activities of the Skin Secretions from Six Common Chinese Amphibians

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 动物毒素室

	Comparative Study of the Biological Activities of the Skin Secretions from Six Common Chinese Amphibians
	Lai R; Zhao Y; Yang DM; Zha HG; Lee WH; Zhang Y[*]
刊名	动物学研究
	2002
卷号	23 期号:2 页码:113-119
关键词	Amphibian Toxicity Antimicrobial Skin secretions Biological activity
ISSN号	0254-5853
其他题名	六种常见两栖类动物皮肤分泌物的生物活性比较
通讯作者	zhangy@mail.kiz.ac.cn
中文摘要	对6种常见两栖类动物皮肤水溶性分泌物的生物学活性与酶学活性进行了研究。其中，红瘰疣螈（Tylototriton verrucosus）、大蹼铃蟾（Bombina maxima）、华西蟾蜍（Bufo andrewsi）皮肤分泌物对小白鼠具有致死毒性，对小白鼠腹腔注射的半数致死剂量分别为11.5、18.8和264mg/kg。而沼哇（Rana nigromaculata）、泽蛙（Rana guentheri）、黑斑蛙（Rana limnocharis）的皮肤分泌物在小白鼠腹腔注射剂量达到500 mg/kg时仍不显示致死毒性。红瘰疣螈、大蹼铃蟾皮肤分泌物的毒性成分为3～60 kDa的多肽与蛋白质。6种两栖类动物皮肤分泌物都具有蛋白酶水解活性与胰蛋白酶蛋白酶抑制活性。红瘰疣螈、大蹼铃蟾和华西蟾蜍皮肤分泌物具有广谱抗菌活性。大蹼铃蟾、华西蟾蜍皮肤分泌物具有肿瘤细胞细胞毒活性。6种皮肤分泌物对哺乳类血液凝固系统无显著影响。仅发现红瘰疣螈皮肤分泌物具有磷脂酶A_2活性。6种皮肤分泌物均无乙酰胆碱脂酶活性。
英文摘要	Water-soluble skin secretions of six common Chinese amphibians were studied for their biological and enzymatic activities.The skin secretions of Tylototriton verrucosus,Bombina maxima,and Bufo andrewsi were found toxic to mice with the intraperitoneal LD[50] of 11.5 mg/kg,18.8 mg/kg,and 264 mg/kg,respectively.No acute lethal toxicities were observed for the skin secretions of Rana nigromaculata,Rana guentheri and Rana limnocharis in a dose up to 500 mg/kg.The lethal toxicities of the skin secretions of T.verrucosus and B.maxima to mice are in the same grade as those of Viperidae snake venoms.The toxic components in T.verrucosus and B.maxima skin secretions are the proteins with molecular weights ranging from 3 to 60 kDa.All the skin secretions had both proteolytic activity and trypsin inhibitory activity.The skin secretions from T.verrucosus,B.maxima and B.andrewsi also displayed wide spectrum antimicrobial activity.On the other hand,the skin secretions from B.andrewsi and B.maxima showed cytotoxicity on human cancer cells.All the six samples had not significant effects on mammalian blood coagulation system.Phospholipase A[2] activity was only found in the skin secretions of T.verrucosus.None of these skin secretions showed acetylcholine esterase activity.
收录类别	其他
资助信息	This work was supported by the grants of“STZ98-3-0 1” ;“Western Light Project”and“Tenth Five Plan”preresearch project from Chinese Academy of Sciences ;and grants from National Natural Science Foundationof China ( 30 170 195 )andYunnan Science and Technology Commission
原文出处	2002232113.pdf
语种	英语
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/1967]
专题	昆明动物研究所_动物毒素室昆明动物研究所_动物活性蛋白多肽组学
作者单位	Kunming Institute of Zoology,the Chinese Academy of Sciences,Kunming,Yunnan 650223,China
推荐引用方式 GB/T 7714	Lai R,Zhao Y,Yang DM,et al. Comparative Study of the Biological Activities of the Skin Secretions from Six Common Chinese Amphibians[J]. 动物学研究,2002,23(2):113-119.
APA	Lai R,Zhao Y,Yang DM,Zha HG,Lee WH,&Zhang Y[*].(2002).Comparative Study of the Biological Activities of the Skin Secretions from Six Common Chinese Amphibians.动物学研究,23(2),113-119.
MLA	Lai R,et al."Comparative Study of the Biological Activities of the Skin Secretions from Six Common Chinese Amphibians".动物学研究 23.2(2002):113-119.