Early embryonic development and transplantation in tree shrews | |
Yan LZ1; Sun B2; Lin Q3; Zheng P1,4; Zhao XD[*]1,2; Lv LB1; Ma YH1; Chen JQ1 | |
刊名 | Zoological Research |
2016 | |
卷号 | 37期号:4页码:252-258 |
关键词 | Tree shrew Embryonic developmental stage Embryo culture |
通讯作者 | zhaoxudong@mail.kiz.ac.cn |
合作状况 | 其它 |
英文摘要 | As a novel experimental animal model, tree shrews have received increasing attention in recent years. Despite this, little is known in regards to the time phases of their embryonic development. In this study, surveillance systems were used to record the behavior and timing of copulations; embryos at different post-copulation stages were collected and cultured in vitro; and the developmental characteristics of both early-stage and in vitro cultured embryos were determined. A total of 163 females were collected following effective copulation, and 150 were used in either unilateral or bilateral oviduct embryo collections, with 307 embryos from 111 females obtained (conception rate=74%). Among them, 237 embryos were collected from 78 females, bilaterally, i.e., the average embryo number per female was 3.04; 172 fertilized eggs collected from 55 females, bilaterally, were cultured for 24-108 h in vitro for developmental observations; finally, 65 embryos from 23 bilateral cases and 70 embryos from 33 unilateral cases were used in embryo transplantation. |
收录类别 | 其他 |
资助信息 | This study was supported by the Breakthrough Project of Strategic Priority Program of the Chinese Academy of Sciences (XDB13000000) |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://159.226.149.26:8080/handle/152453/10354] |
专题 | 昆明动物研究所_哺乳动物胚胎发育 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_肿瘤干细胞生物学 昆明动物研究所_中国科学院昆明灵长类研究中心 |
作者单位 | 1.Kunming Primate Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China 2.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223 China 3.Yunnan University of Traditional Chinese Medicine, Kunming Yunnan 650500 China 4.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223 China |
推荐引用方式 GB/T 7714 | Yan LZ,Sun B,Lin Q,et al. Early embryonic development and transplantation in tree shrews[J]. Zoological Research,2016,37(4):252-258. |
APA | Yan LZ.,Sun B.,Lin Q.,Zheng P.,Zhao XD[*].,...&Chen JQ.(2016).Early embryonic development and transplantation in tree shrews.Zoological Research,37(4),252-258. |
MLA | Yan LZ,et al."Early embryonic development and transplantation in tree shrews".Zoological Research 37.4(2016):252-258. |
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