云南水牛的同期发情、超数排卵和胚胎移植试验

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	云南水牛的同期发情、超数排卵和胚胎移植试验
	和占星[*]1; 和协超 2; 创向辉 5; 尹安强 3; 黄晓松 5; 罗在仁 3; 朱芳贤 1; 铃木达行 4
刊名	动物学研究
	2005
卷号	26 期号:1 页码:106-111
关键词	水牛同期发情超数排卵胚胎移植云南氯前列烯醇
通讯作者	hezx81@public.km.yn.cn
中文摘要	为探讨水牛胚胎移植的效果,于2002年对云南水牛进行了胚胎移植试验:①用国产氯前列烯醇(PG)0.6 mg/头·次处理供、受体水牛的同期发情率和可用率分别为43.33%(13/30)和16.67%(5/30);同期发情率经产水牛高于青年水牛(P=0.086),杂交水牛高于德宏水牛(P=0.153),体重401～530 kg水牛显著高于体重300～400 kg水牛(P<0.05);发情明显水牛的可用率极显著高于发情不明显的水牛(P<0.01).②选用河流型摩拉水牛与沼泽型德宏水牛的杂交一代5头为供体,分进口激素组(n=2)和国产激素组(n=3)进行超数排卵,共有2头获9枚胚胎;进口激素组供体的平均获胚数和可移植胚数分别为2.0枚和1.5枚,比国产激素组分别多0.33枚(P=0.454)和1.17枚(P=0.288).③所获4枚可用鲜胚分别移植3头受体,结果90 d的妊娠率为33.33%,但最终无一头产犊.试验结果表明使用进口FSH 24mg+PG (Lutalyse○R)35 mg和国产FSH 11 mg+PG 0.8 mg对水牛超数排卵有效,同时提示需要足够数量的受体,从中选用发情表现明显、黄体发育良好的进行胚胎移植,才能取得良好效果.
英文摘要	To investigate the effect of embryo transfer in buffaloes, buffaloes of Yunnan were experimented in 2002. Results showed:(1)Synchronous estrus and usable rates of donors and recipients administered with cloprostenol (PG) 0.6 mg/head,injection once,were 43.33% (13/30) and 16.67% (5/30). Especially,the synchronous estrus rate was higher in paritys than in heifer s (P=0.086),higher in crossbreds than in Dehong Buffalos (P=0.153),and higher in weight from 401 to 530 kg of buffalo cows than from 300 to 400 kg (P<0.05);And the usable rate of buffaloes with evidence estrus behavior was also higher than that of buffaloes with lack of evidence estrus (P<0.01).(2)Five donors crossbred F[1] (Murrah×Dehong buffalo) were picked out from buffaloes of synchronous estrus and divided into two groups,I.e. import hormone group (n=2) and native hormone group (n=3),which were dealt with superovulation. The results showed that 2 out of 5 buffaloes gave 9 embryos,and average no. of recovered embryos and no. of transferable embryos in the import hormone were 2.0 and 1.5,and higher than those in the native hormone,which were 1.67 (P=0.454) and 0.33 (P=0.288),respectively.(3)Four fresh embryos were transferred into 3 receipts,thereinto the rate of 90-day conception was 33.33%,but finally none gave birth to a calf. The experiment results indicate that it is in effect for superovulation of buffaloes with administered 24 mg of import FSH and plus PG (Lutalyse[R]) 35 mg and the same as 11 mg of native FSH and plus PG 0.8 mg,meanwhile suggest that it is practicable if the receipt buffaloes are enough for synchronous estrus,then the cows with evidence estrus behavior and good CL checked can be selected for transferring embryos.
收录类别	其他
资助信息	云南省重点实验室基金，国家外专局资助项目
语种	中文
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10281]
专题	昆明动物研究所_哺乳动物胚胎发育
作者单位	1.云南省肉牛和牧草研究中心,云南,昆明,650212 2.中国科学院昆明动物研究所,云南,昆明,650223 3.盈江县畜牧局,云南,盈江,679300 4.山口大学,日本,山口,753-8515 5.德宏州畜牧兽医站,云南,潞西,678400
推荐引用方式 GB/T 7714	和占星[*],和协超,创向辉,等. 云南水牛的同期发情、超数排卵和胚胎移植试验[J]. 动物学研究,2005,26(1):106-111.
APA	和占星[*].,和协超.,创向辉.,尹安强.,黄晓松.,...&铃木达行.(2005).云南水牛的同期发情、超数排卵和胚胎移植试验.动物学研究,26(1),106-111.
MLA	和占星[*],et al."云南水牛的同期发情、超数排卵和胚胎移植试验".动物学研究 26.1(2005):106-111.