mRNA-Seq and MicroRNA-Seq Whole-Transcriptome Analyses of Rhesus Monkey Embryonic Stem Cell Neural Differentiation Revealed the Potential Regulators of Rosette Neural Stem Cells | |
Zhao YQ1; Ji S1,2,3; Wang JK4; Huang JF[*]1,5; Zheng P[*]1,2 | |
刊名 | DNA RESEARCH |
2014 | |
卷号 | 21期号:5页码:541-554 |
关键词 | rhesus monkeys embryonic stem cells neural differentiation transcriptome microRNAomes |
通讯作者 | huangjf@mail.kiz.ac.cn ; zhengp@mail.kiz.ac.cn |
英文摘要 | Rosette neural stem cells (R-NSCs) represent early stage of neural development and possess full neural differentiation and regionalization capacities. R-NSCs are considered as stem cells of neural lineage and have important implications in the study of neurogenesis and cell replacement therapy. However, the molecules regulating their functional properties remain largely unknown. Rhesus monkey is an ideal model to study human neural degenerative diseases and plays intermediate translational roles as therapeutic strategies evolved from rodent systems to human clinical applications. In this study, we derived R-NSCs from rhesus monkey embryonic stem cells (ESCs) and systematically investigated the unique expressions of mRNAs, microRNAs (miRNAs), and signalling pathways by genome-wide comparison of the mRNA and miRNA profilings of ESCs, R-NSCs at early (R-NSCP1) and late (R-NSCP6) passages, and neural progenitor cells. Apart from the R-NSCP1-specific protein-coding genes and miRNAs, we identified several pathways including Hedgehog and Wnt highly activated in R-NSCP1. The possible regulatory interactions among the miRNAs, protein-coding genes, and signalling pathways were proposed. Besides, many genes with alternative splicing switch were identified at R-NSCP1. These data provided valuable resource to understand the regulation of early neurogenesis and to better manipulate the R-NSCs for cell replacement therapy. |
收录类别 | SCI |
资助信息 | This work was supported by the National Key Basic Research Program of China (grant number 2011CBA 01101). |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://159.226.149.26:8080/handle/152453/9257] |
专题 | 昆明动物研究所_结构生物信息学 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_哺乳动物胚胎发育 |
作者单位 | 1.State Key Laboratory of Genetic Resources and Evolution,KunmingInstituteofZoology,ChineseAcademyofSciences,32 East Jiaochang Road, Kunming, Yunnan 650223, China 2.Yunnan Key Laboratory of Animal Reproductive Biology, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 East Jiaochang Road, Kunming, Yunnan 650223, China 3.University of Chinese Academy of Sciences, Beijing, China 4.Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA 5.Kunming Institute of Zoology, Chinese University of Hongkong Joint Research Center for Bio-resources and Human Disease Mechanisms, Kunming, Yunnan 650223, China |
推荐引用方式 GB/T 7714 | Zhao YQ,Ji S,Wang JK,et al. mRNA-Seq and MicroRNA-Seq Whole-Transcriptome Analyses of Rhesus Monkey Embryonic Stem Cell Neural Differentiation Revealed the Potential Regulators of Rosette Neural Stem Cells[J]. DNA RESEARCH,2014,21(5):541-554. |
APA | Zhao YQ,Ji S,Wang JK,Huang JF[*],&Zheng P[*].(2014).mRNA-Seq and MicroRNA-Seq Whole-Transcriptome Analyses of Rhesus Monkey Embryonic Stem Cell Neural Differentiation Revealed the Potential Regulators of Rosette Neural Stem Cells.DNA RESEARCH,21(5),541-554. |
MLA | Zhao YQ,et al."mRNA-Seq and MicroRNA-Seq Whole-Transcriptome Analyses of Rhesus Monkey Embryonic Stem Cell Neural Differentiation Revealed the Potential Regulators of Rosette Neural Stem Cells".DNA RESEARCH 21.5(2014):541-554. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论