A novel fusion gene, TRIMS-Cyclophilin A in the pig-tailed macaque determines its susceptibility to HIV-1 infection

CORC > 昆明动物研究所 > 昆明动物研究所 > 遗传资源与进化国家重点实验室 > 比较基因组学

	A novel fusion gene, TRIMS-Cyclophilin A in the pig-tailed macaque determines its susceptibility to HIV-1 infection
	Liao CH 1,2,4; Kuang YQ 1,3,4; Liu HL 1,3,4; Zheng YT[]1,3; Su B[]1,2
刊名	AIDS
	2007
卷号	21 期号:增刊8 页码:S19-S26
关键词	tRIM5 alpha Cyclophilin A fusion gene HIV-1 restriction factor pig-tailed macaque
通讯作者	zhengyt@mail.kiz.ac.cn ; sub@mail.kiz.ac.cn
合作状况	其它
英文摘要	Objective: In Old World monkeys, the tripartite motif Sec (TRIM5 alpha) protein confers resistance to HIV-1 infection following virus entry into host cells. However, the pig-tailed macaque (Macaca nemestrina) is an exception and is susceptible to HIV-1 infection. This study dissects the molecular mechanism of the pig-tailed macaque's susceptibility to HIV-1 infection. Methods: Genomic sequencing and expression analysis of the TRIM5a gene was conducted in the pig-tailed macaque. A novel TRIMS-Cyclophilin A fusion gene isoform was identified and subsequently cloned into the pcDNA3.1(+) expression vector. This construct was transfected into HeLa-T4 or HeLa cells which were then infected with the HIV-1(IIIB) or HIV-GFP-VSVG pseudotyped virus, to examine the effects of the TRIMS-Cyclophilin A fusion protein on HIV-1 infection. Results: A novel TRIMS-Cyclophilin A fusion gene (rnnTRIMCyp) in the pig-tailed macaque was found and its fusion pattern is different from the known fusion gene in the owl monkey (owITRIMCyp). TRIMCyp protein expression in transfected cells was confirmed by western blotting. The tests using HIV-1(IIIB) and HIV-GFP-VSVG pseudotyped virus indicated that mnTRIMCyp did not inhibit HIV-1 replication at various multiplicities of infection. Conclusions: The mnTRIMCyp fusion protein does not restrict replication of HIV-1, which provides a potential molecular mechanism that might explain why the pig-tailed macaque is prone to HIV-1 infection, the only known exception in Old World monkeys. (c) 2007 Wolters Kluwer Health-Lippincott Williams & Wilkins.
收录类别	SCI
资助信息	This work was supported in part by grants from the CAS (KSCX1-YW-R-34, KSCX1-YW-R- 15), the NSFC (30630013, 30525028, 30440018, 3 0 4 7 1 6 0 5 , 3 0 6 7 1 9 6 0 ) , 9 7 3 p r o g r a m (2006CB701506, 2006CB504200, 2006CB504300, 2007CB815705), Key Technology R & D program of China (2004BA719A14), and Yunnan (2004NG12).
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10517]
专题	昆明动物研究所_比较基因组学昆明动物研究所_动物模型与人类重大疾病机理重点实验室昆明动物研究所_遗传资源与进化国家重点实验室昆明动物研究所_分子免疫药理学昆明动物研究所_中国科学院昆明灵长类研究中心
作者单位	1.Kunming Primate Research Center,Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 2.State Key Laboratory of Genetic Resources and Evolution,Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 3.Laboratory of Molecular Immunopharmacology, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 4.Graduate School of Chinese Academy of Sciences, Beijing, China
推荐引用方式 GB/T 7714	Liao CH,Kuang YQ,Liu HL,et al. A novel fusion gene, TRIMS-Cyclophilin A in the pig-tailed macaque determines its susceptibility to HIV-1 infection[J]. AIDS,2007,21(增刊8):S19-S26.
APA	Liao CH,Kuang YQ,Liu HL,Zheng YT[],&Su B[].(2007).A novel fusion gene, TRIMS-Cyclophilin A in the pig-tailed macaque determines its susceptibility to HIV-1 infection.AIDS,21(增刊8),S19-S26.
MLA	Liao CH,et al."A novel fusion gene, TRIMS-Cyclophilin A in the pig-tailed macaque determines its susceptibility to HIV-1 infection".AIDS 21.增刊8(2007):S19-S26.