Effects of salinization and crude oil contamination on soil bacterial community structure in the Yellow River Delta region, China

	Effects of salinization and crude oil contamination on soil bacterial community structure in the Yellow River Delta region, China
	Y. C. Gao; J. N. Wang; S. H. Guo; Y. L. Hu; T. T. Li; R. Mao; D. H. Zeng
刊名	Applied Soil Ecology
	2015
卷号	86 页码:165-173
通讯作者	Zeng, D. H.
中文摘要	Soil salinization is a predominant environmental character in oil fields, especially in coastal regions. However, information about the coupling effect of crude oil contamination and salinization on soil biological characteristics is lacking. Therefore, the objective of this study was to examine soil bacterial community changes in response to different gradients of salinity and total petroleum hydrocarbon (TPH) concentration. Fifteen soil samples collected from the Yellow River Delta region of China with different gradients of salinity and TPH concentration were used for analyzing soil physicochemical properties, microbial biomass and denaturing gradient gel electrophoresis (DGGE) profiles. The results showed that salinity negatively affected soil microbial biomass carbon (MBC) and microbial biomass nitrogen (MBN), but little affected bacterial Shannon and evenness indices. TPH concentration was correlated negatively with soil MBC, positively with MBN and Shannon index, but had no effect on evenness index. Canonical correspondence analysis showed that salinity and TPH concentration were the main factors causing the shift of soil bacterial community structure. Soil salinity had a suppress effect on most bacterial populations without changing their dominance, while soil TPH influenced the bacterial diversity selectively. By extraction of main bacterial clusters from the dendrogram tree of DGGE profiles, the most active bacterial species involved in the shift of bacterial community structure were identified under the single or dual stresses of salinization and oil contamination. Actinobacteria, gamma-Proteobacteria, Firmicutes, Deinococcus-Thermus and some unclassified bacteria were the dominant bacteria participating in crude oil degradation in dual stresses of salinization and oil contamination. Our results provide new insight and useful information in the screening of cultivable bacteria for bioremediation of crude oil contaminated saline. (C) 2014 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://159.226.123.10/handle/131322/6472]
专题	东北地理与农业生态研究所_合作研究组_期刊论文
推荐引用方式 GB/T 7714	Y. C. Gao,J. N. Wang,S. H. Guo,et al. Effects of salinization and crude oil contamination on soil bacterial community structure in the Yellow River Delta region, China[J]. Applied Soil Ecology,2015,86:165-173.
APA	Y. C. Gao.,J. N. Wang.,S. H. Guo.,Y. L. Hu.,T. T. Li.,...&D. H. Zeng.(2015).Effects of salinization and crude oil contamination on soil bacterial community structure in the Yellow River Delta region, China.Applied Soil Ecology,86,165-173.
MLA	Y. C. Gao,et al."Effects of salinization and crude oil contamination on soil bacterial community structure in the Yellow River Delta region, China".Applied Soil Ecology 86(2015):165-173.