Sequence-specific RNA Photocleavage by Single-stranded DNA in Presence of Riboflavin

	Sequence-specific RNA Photocleavage by Single-stranded DNA in Presence of Riboflavin
	Zhao, Yongyun; Chen, Gangyi; Yuan, Yi; Li, Na; Dong, Juan; Huang, Xin; Cui, Xin; Tang, Zhuo
刊名	SCIENTIFIC REPORTS
	2015
卷号	5 页码:-
关键词	IN-VITRO SELECTION U BASE-PAIRS ARTIFICIAL RIBONUCLEASES HAMMERHEAD RIBOZYME CLEAVAGE SITE DEOXYRIBOZYMES MIMICS
通讯作者	Tang, Z (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Peoples R China.
产权排序	1
合作状况	国内
英文摘要	Constant efforts have been made to develop new method to realize sequence-specific RNA degradation, which could cause inhibition of the expression of targeted gene. Herein, by using an unmodified short DNA oligonucleotide for sequence recognition and endogenic small molecue, vitamin B2 (riboflavin) as photosensitizer, we report a simple strategy to realize the sequence-specific photocleavage of targeted RNA. The DNA strand is complimentary to the target sequence to form DNA/RNA duplex containing a G.U wobble in the middle. The cleavage reaction goes through oxidative elimination mechanism at the nucleoside downstream of U of the G.U wobble in duplex to obtain unnatural RNA terminal, and the whole process is under tight control by using light as switch, which means the cleavage could be carried out according to specific spatial and temporal requirements. The biocompatibility of this method makes the DNA strand in combination with riboflavin a promising molecular tool for RNA manipulation.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
语种	英语
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/27563]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Zhao, Yongyun,Chen, Gangyi,Yuan, Yi,et al. Sequence-specific RNA Photocleavage by Single-stranded DNA in Presence of Riboflavin[J]. SCIENTIFIC REPORTS,2015,5:-.
APA	Zhao, Yongyun.,Chen, Gangyi.,Yuan, Yi.,Li, Na.,Dong, Juan.,...&Tang, Zhuo.(2015).Sequence-specific RNA Photocleavage by Single-stranded DNA in Presence of Riboflavin.SCIENTIFIC REPORTS,5,-.
MLA	Zhao, Yongyun,et al."Sequence-specific RNA Photocleavage by Single-stranded DNA in Presence of Riboflavin".SCIENTIFIC REPORTS 5(2015):-.