Sensitivity of transmitted and founder human immunodeficiency virus type 1 envelopes to carbohydrate-binding agents griffithsin, cyanovirin-N and Galanthus nivalis agglutinin

	Sensitivity of transmitted and founder human immunodeficiency virus type 1 envelopes to carbohydrate-binding agents griffithsin, cyanovirin-N and Galanthus nivalis agglutinin
	Hu, Bodan; Du, Tao; Li, Chang; Luo, Sukun; Liu, Yalan; Huang, Xin; Hu, Qinxue
刊名	JOURNAL OF GENERAL VIROLOGY
	2015
卷号	96 页码:3660-3666
关键词	HIV-1 GP120 INACTIVATING PROTEIN GLYCOSYLATION SITES GLYCOPROTEIN GP120 GLYCAN DELETIONS PLANT-LECTINS MANNOSE RESISTANCE TRANSMISSION INFECTIONS
通讯作者	Hu, QX (reprint author), Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China. ; Hu, QX (reprint author), Univ London, Inst Infect & Immun, London SW17 0RE, England.
产权排序	3
合作状况	国际
英文摘要	Human immunodeficiency virus type 1 (HIV-1) transmission often results from infection by a single transmitted/founder (T/F) virus. Here, we investigated the sensitivity of T/F HIV-1 envelope glycoproteins (Envs) to microbicide candidate carbohydrate-binding agents (CBAs) griffithsin (GRFT), cyanovirin-N (CV-N) and Galanthus nivalis agglutinin (GNA), showing that T/F Envs demonstrated different sensitivity to CBAs, with IC50 values ranging from 0.006 +/- 0.0003 to > 10 nM for GRFT, from 0.6 +/- 0.2 to 28.9 +/- 2.9 nM for CV-N and from 1.3 +/- 0.2 to > 500 nM for GNA. We further revealed that deglycosylation at position 295 or 448 decreased the sensitivity of T/F Env to GRFT, and at 339 to both CV-N and GNA. Mutation of all the three glcyans rendered a CBA-sensitive T/F Env largely resistant to GRFT, indicating that the sensitivity of T/F Env to GRFT is mainly determined by glycans at 295, 339 and 448. Our study identified specific T/F Env residues associated with CBA sensitivity.
学科主题	Biotechnology & Applied Microbiology; Virology
类目[WOS]	Biotechnology & Applied Microbiology ; Virology
语种	英语
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/27554]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Hu, Bodan,Du, Tao,Li, Chang,et al. Sensitivity of transmitted and founder human immunodeficiency virus type 1 envelopes to carbohydrate-binding agents griffithsin, cyanovirin-N and Galanthus nivalis agglutinin[J]. JOURNAL OF GENERAL VIROLOGY,2015,96:3660-3666.
APA	Hu, Bodan.,Du, Tao.,Li, Chang.,Luo, Sukun.,Liu, Yalan.,...&Hu, Qinxue.(2015).Sensitivity of transmitted and founder human immunodeficiency virus type 1 envelopes to carbohydrate-binding agents griffithsin, cyanovirin-N and Galanthus nivalis agglutinin.JOURNAL OF GENERAL VIROLOGY,96,3660-3666.
MLA	Hu, Bodan,et al."Sensitivity of transmitted and founder human immunodeficiency virus type 1 envelopes to carbohydrate-binding agents griffithsin, cyanovirin-N and Galanthus nivalis agglutinin".JOURNAL OF GENERAL VIROLOGY 96(2015):3660-3666.