Preparative Separation of N-Feruloyl Serotonin and N-(p-Coumaroyl) Serotonin from Safflower Seed Meal Using High-Speed Counter-Current Chromatography

	Preparative Separation of N-Feruloyl Serotonin and N-(p-Coumaroyl) Serotonin from Safflower Seed Meal Using High-Speed Counter-Current Chromatography
	Zhang, Qiulong 1,2; Hu, Na 1,2; Li, Wencong 1,2; Ding, Chenxi 3; Ma, Tao 1,2; Bai, Bo 1; Wang, Honglun 1; Suo, Yourui 1; Wang, Xiaoyan 1; Ding, Chenxu 1,4,5
刊名	journal of chromatographic science
	2015-09-01
卷号	53 期号:8 页码:1341-1345
英文摘要	high-speed counter-current chromatography (hsccc) was successfully applied for the preparative separation and purification of n-feruloyl serotonin (nf) and n-(p-coumaroyl) serotonin (np) from safflower seed meal. after the measurement of partition coefficient of the two target compounds in the two-phase solvent systems, the hsccc was performed well with a two-phase solvent system composed of chcl3-methanol-0.1 m hcl at a volume ratio of 1 : 1 : 1, v/v. the upper phase was used as stationary phase and the lower phase was used as mobile phase. under the optimized condition, 7.5 mg nf and 6.9 mg np were separated from 40 mg crude sample with the purity of 98.8 and 97.3%, respectively. the structures of the isolated compounds were identified by h-1 nmr and c-13 nmr.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	carthamus-tinctorius l ; conjugated serotonins ; derivatives ; inhibition ; plant
收录类别	SCI
语种	英语
WOS记录号	WOS:000366629000016
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/6510]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qinghai Red Cross Hosp, Xining 810099, Peoples R China 4.Qinghai Nationalities Univ, Xining 810007, Peoples R China 5.Key Lab Plateau Crop Germplasm Innovat & Utilizat, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Qiulong,Hu, Na,Li, Wencong,et al. Preparative Separation of N-Feruloyl Serotonin and N-(p-Coumaroyl) Serotonin from Safflower Seed Meal Using High-Speed Counter-Current Chromatography[J]. journal of chromatographic science,2015,53(8):1341-1345.
APA	Zhang, Qiulong.,Hu, Na.,Li, Wencong.,Ding, Chenxi.,Ma, Tao.,...&Ding, Chenxu.(2015).Preparative Separation of N-Feruloyl Serotonin and N-(p-Coumaroyl) Serotonin from Safflower Seed Meal Using High-Speed Counter-Current Chromatography.journal of chromatographic science,53(8),1341-1345.
MLA	Zhang, Qiulong,et al."Preparative Separation of N-Feruloyl Serotonin and N-(p-Coumaroyl) Serotonin from Safflower Seed Meal Using High-Speed Counter-Current Chromatography".journal of chromatographic science 53.8(2015):1341-1345.