Isolation and Identification of Saponins from the Natural Pasturage Asterothamnus centrali-asiaticus Employing Preparative Two-Dimensional Reversed-Phase Liquid Chromatography/Hydrophilic Interaction Chromatography

	Isolation and Identification of Saponins from the Natural Pasturage Asterothamnus centrali-asiaticus Employing Preparative Two-Dimensional Reversed-Phase Liquid Chromatography/Hydrophilic Interaction Chromatography
	Wang, Yan-Ming 1,2,4; Zhao, Jian-Qiang 3,4; Yang, Jun-Li 1,2; Tao, Yan-Duo 3; Mei, Li-Juan 3; Shi, Yan-Ping 1,2
刊名	journal of agricultural and food chemistry
	2016-06-22
卷号	64 期号:24 页码:4950-4957
关键词	Asterothamnus centrali-asiaticus triterpenoid saponins 2D RPLC/HILIC cytotoxicity
英文摘要	asterothamnus centrali-asiaticus, a kind of characteristic shrub abundant in grassland and desert areas, has been used as forage fodder for camels and goats in central asia, and this plant also plays a critical role in the maintenance of desert grassland ecosystems as a result of its tolerance to poor soils and sand burial. however, its chemical composition has been rarely reported. in this study, phytochemical investigation of this pasturage was performed and three new triterpenoid saponins (1-3) were isolated together with nine known triterpenoid saponins (4-12) using preparative two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography (2d rplc/hilic). their structures were elucidated via diverse spectroscopic analyses, including infrared (ir) spectrometry, high-resolution electrospray ionization mass spectrometry (hr-esims), and one-dimensional (1d) and 2d nuclear magnetic resonance (nmr). all isolated triterpenoid saponins (1-12) were reported from this genus for the first time, and they were further evaluated for their cytotoxicity against four cancer cell lines (a549, hepg2, mgc-803, and mfc), which indicated that compound 11 showed potent cytotoxicity against the hepg2 cell line, with an ic50 value of 6.85 mu g/ml.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	agriculture, multidisciplinary ; chemistry, applied ; food science & technology
研究领域[WOS]	agriculture ; chemistry ; food science & technology
关键词[WOS]	hydrophilic interaction chromatography ; triterpenoid saponins ; platycodon-grandiflorum ; aster-auriculatus ; purification ; separation ; bufadienolides ; ginsenosides ; column ; roots
收录类别	SCI
语种	英语
WOS记录号	WOS:000378584300016
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/6481]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Key Lab Nat Med Gansu Prov, Lanzhou Inst Chem Phys, Lanzhou 730000, Gansu, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yan-Ming,Zhao, Jian-Qiang,Yang, Jun-Li,et al. Isolation and Identification of Saponins from the Natural Pasturage Asterothamnus centrali-asiaticus Employing Preparative Two-Dimensional Reversed-Phase Liquid Chromatography/Hydrophilic Interaction Chromatography[J]. journal of agricultural and food chemistry,2016,64(24):4950-4957.
APA	Wang, Yan-Ming,Zhao, Jian-Qiang,Yang, Jun-Li,Tao, Yan-Duo,Mei, Li-Juan,&Shi, Yan-Ping.(2016).Isolation and Identification of Saponins from the Natural Pasturage Asterothamnus centrali-asiaticus Employing Preparative Two-Dimensional Reversed-Phase Liquid Chromatography/Hydrophilic Interaction Chromatography.journal of agricultural and food chemistry,64(24),4950-4957.
MLA	Wang, Yan-Ming,et al."Isolation and Identification of Saponins from the Natural Pasturage Asterothamnus centrali-asiaticus Employing Preparative Two-Dimensional Reversed-Phase Liquid Chromatography/Hydrophilic Interaction Chromatography".journal of agricultural and food chemistry 64.24(2016):4950-4957.