Extraction and Separation of Vitisin D, Ampelopsin B and cis-Vitisin A from Iris lactea Pall. var. chinensis (Fisch.) Koidz by Alkaline Extraction-Acid Precipitation and High-Speed Counter-Current Chromatography

	Extraction and Separation of Vitisin D, Ampelopsin B and cis-Vitisin A from Iris lactea Pall. var. chinensis (Fisch.) Koidz by Alkaline Extraction-Acid Precipitation and High-Speed Counter-Current Chromatography
	Lv, Huanhuan 1,2; Zhou, Wenna 1,2; Wang, Xiaoyan 1; Wang, Zhenhua 1,3; Suo, Yourui 1; Wang, Honglun 1
刊名	journal of chromatographic science
	2016-05-01
卷号	54 期号:5 页码:744-751
英文摘要	naturally occurring oligostilbenes are receiving more attention because they exhibit several beneficial effects for health, including hepatoprotective, antitumor, anti-adipogenic, antioxidant, antiaging, anti-inflammatory, anti-microbial, antiviral, immunosuppressive and neuroprotective activities. thus, they could be of some potentially therapeutic values for several diseases. in this study, we adopted the alkaline extraction-acid precipitation (aeap) method for extraction of oligostilbenes from the seed kernel of iris lactea. then, the high-speed counter-current chromatography (hsccc) was used for preparative isolation and purification of oligostilbenes from the aeap extracts. finally, three oligostilbenes, namely vitisin d (73 mg), ampelopsin b (25 mg) and cis-vitisin a (16 mg), were successfully fractionated by hsccc with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (2:5:3:6, v/v/v/v) from 300 mg of the aeap extracts in similar to 190 min. the purities of the three isolated oligostilbenes were all over 95.0% as analyzed by high performance liquid chromatography. they all were isolated from i. lactea for the first time. the method of aeap for the preparation of the oligostilbene-enriched crude sample was simple, and the hsccc technique for the isolation and purification of oligostilbenes was efficient.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	oligostilbenes ; amurensis ; stem ; leaf ; stilbenes ; antioxidant ; resveratrol ; cells ; mice
收录类别	SCI
语种	英语
WOS记录号	WOS:000381702100009
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/6442]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Yantai Univ, Yantai 264005, Peoples R China
推荐引用方式 GB/T 7714	Lv, Huanhuan,Zhou, Wenna,Wang, Xiaoyan,et al. Extraction and Separation of Vitisin D, Ampelopsin B and cis-Vitisin A from Iris lactea Pall. var. chinensis (Fisch.) Koidz by Alkaline Extraction-Acid Precipitation and High-Speed Counter-Current Chromatography[J]. journal of chromatographic science,2016,54(5):744-751.
APA	Lv, Huanhuan,Zhou, Wenna,Wang, Xiaoyan,Wang, Zhenhua,Suo, Yourui,&Wang, Honglun.(2016).Extraction and Separation of Vitisin D, Ampelopsin B and cis-Vitisin A from Iris lactea Pall. var. chinensis (Fisch.) Koidz by Alkaline Extraction-Acid Precipitation and High-Speed Counter-Current Chromatography.journal of chromatographic science,54(5),744-751.
MLA	Lv, Huanhuan,et al."Extraction and Separation of Vitisin D, Ampelopsin B and cis-Vitisin A from Iris lactea Pall. var. chinensis (Fisch.) Koidz by Alkaline Extraction-Acid Precipitation and High-Speed Counter-Current Chromatography".journal of chromatographic science 54.5(2016):744-751.