The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through TNF pathway in oyster Crassostrea gigas

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	The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through TNF pathway in oyster Crassostrea gigas
	Liu, Zhaoqun1,3 ; Zhou, Zhi 1; Wang, Lingling 2; Dong, Wenjing1 ; Qiu, Limei 1; Song, Linsheng 2
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2016-12-01
卷号	65 页码:139-148
关键词	Crassostrea gigas Acetylcholine Muscarinic acetylcholine receptor TNF pathway Immune regulation
英文摘要	Muscarinic receptors, which selectively take muscarine as their ligand, are critical for the immunological and physiological processes in animals. In the present study, the open region frame (ORF) of a homologue of muscarinic acetylcholine (ACh) receptor (mAChR) was amplified from oyster Crassostrea gigas (named as CgmAChR-1), whose full length was 1983 bp and the protein it encoded contained 660 amino acids with a seven transmembrane region. Phylogeny analysis suggested that CgmAChR-1 shared homology with M-5 muscarinic receptor found in invertebrates including Habropoda laboriosa, Acromyrmex echinatior and Echinococcus granulosus. After cell transfection of CgrnAChR-1 into HEK293T cells and ACh incubation, the level of intracellular Ca2+ and CAMP increased significantly (p < 0.05). Such trend could be reverted with the addition of M-3 and M-5 muscarinic receptor antagonists DAMP and DAR. The CgmAChR-1 transcripts were ubiquitously detectable in seven different tissues with the maximal expression level in adductor muscle. When the oysters received LPS stimulation, CgmAChR-1 mRNA expression in haemocyte was increased to the highest level (6.05-fold, p < 0.05) at 24 h, while blocking CgmAChR-1 using receptor antagonists before LPS stimulation promoted the expression of oyster TNF, resulting in the increase of haemocyte apoptosis index. These results suggested that CgmAChR-1 was the key molecule in cholinergic neuroendocrine-immune system contributing to the regulation of TNF expression and apoptosis process. (C) 2016 Elsevier Ltd. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000384388400016
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/135892]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Zhaoqun,Zhou, Zhi,Wang, Lingling,et al. The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through TNF pathway in oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,65:139-148.
APA	Liu, Zhaoqun,Zhou, Zhi,Wang, Lingling,Dong, Wenjing,Qiu, Limei,&Song, Linsheng.(2016).The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through TNF pathway in oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,65,139-148.
MLA	Liu, Zhaoqun,et al."The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through TNF pathway in oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 65(2016):139-148.