A highly sensitive method for analyzing marker phytoplankton pigments: Ultra-high-performance liquid chromatography-tandem triple quadrupole mass spectrometry

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	A highly sensitive method for analyzing marker phytoplankton pigments: Ultra-high-performance liquid chromatography-tandem triple quadrupole mass spectrometry
	Zhang, JJ; Liu, DY; Cao, P; Wang, YJ; Keesing, JK; Li, JH; Chen, LX
刊名	LIMNOLOGY AND OCEANOGRAPHY-METHODS
	2016-10-01
卷号	14 期号:10 页码:623-636
ISSN号	1541-5856
通讯作者	Liu, DY (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Shandong, Peoples R China. ; Liu, DY (reprint author), Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China. Email:dyliu@yic.ac.cn
产权排序	[Zhang, Jingjing; Liu, Dongyan; Wang, Yujue; Li, Jinhua; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Shandong, Peoples R China; [Zhang, Jingjing; Liu, Dongyan; Wang, Yujue; Li, Jinhua; Chen, Lingxin] Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China; [Zhang, Jingjing] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Cao, Peng] Yantai Entry Exit Inspect & Quarantine Bur, Yantai 264000, Shandong, Peoples R China; [Keesing, John K.] CSIRO Oceans & Atmosphere, Private Bag 5, Wembley, WA 6913, Australia
英文摘要	A sensitive and specific ultra-high-performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-MS/MS) method was developed for the purpose of decreasing the detection limit of quantification (LOQ) and reducing sample analysis time. In total, 14 marker pigments were determined and validated using an internal standard (IS) method with trans-8'-Apo-beta-carotenal as the IS. Electrospray ionization in positive mode was applied for the detection of pigments. The 14 types of pigments were separated in less than 15 min and presented good linearity, with the coefficients ranging from 0.9915 to 0.9991 using a UHPLC C8 column. The LOQ attained for 14 pigments were from 0.005 ng mL(-1) to 0.500 ng mL(-1), which enabled quantification of the pigments in seawater at ppt level. Both intra-day and inter-day precisions were less than 15%. The method was validated using seawater samples and the recovery of the analytes ranged from 62.8% to 120.2% at two spiked concentrations. Compared with previous methods, UHPLC-MS/MS method improved the efficiency of qualitative analysis and significantly decreased the detection LOQ for analyzing marker phytoplankton pigments at trace/ultra-trace levels in complex matrices.
研究领域[WOS]	Marine & Freshwater Biology ; Oceanography
关键词[WOS]	CONTAINING MOBILE PHASES ; MARINE-PHYTOPLANKTON ; EMILIANIA-HUXLEYI ; ANTARCTIC LAKE ; HPLC METHOD ; CHLOROPHYLLS ; CAROTENOIDS ; SEPARATION ; RESOLUTION ; DISTRIBUTIONS
收录类别	SCI
语种	英语
WOS记录号	WOS:000386966700001
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/17471]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室烟台海岸带研究所_近岸生态与环境实验室
推荐引用方式 GB/T 7714	Zhang, JJ,Liu, DY,Cao, P,et al. A highly sensitive method for analyzing marker phytoplankton pigments: Ultra-high-performance liquid chromatography-tandem triple quadrupole mass spectrometry[J]. LIMNOLOGY AND OCEANOGRAPHY-METHODS,2016,14(10):623-636.
APA	Zhang, JJ.,Liu, DY.,Cao, P.,Wang, YJ.,Keesing, JK.,...&Chen, LX.(2016).A highly sensitive method for analyzing marker phytoplankton pigments: Ultra-high-performance liquid chromatography-tandem triple quadrupole mass spectrometry.LIMNOLOGY AND OCEANOGRAPHY-METHODS,14(10),623-636.
MLA	Zhang, JJ,et al."A highly sensitive method for analyzing marker phytoplankton pigments: Ultra-high-performance liquid chromatography-tandem triple quadrupole mass spectrometry".LIMNOLOGY AND OCEANOGRAPHY-METHODS 14.10(2016):623-636.