Enantioselective Resolution of (+/-)-1-Phenylethanol and (+/-)-1-Phenylethyl Acetate by a Novel Esterase from Bacillus sp SCSIO 15121

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	Enantioselective Resolution of (+/-)-1-Phenylethanol and (+/-)-1-Phenylethyl Acetate by a Novel Esterase from Bacillus sp SCSIO 15121
	Liang, Jiayuan; Zhang, Yun; Sun, Aijun; Deng, Dun; Hu, Yunfeng
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2016
卷号	178 期号:3 页码:558-575
关键词	Esterase Kinetic resolution Chiral 1-phenylethanol Chiral 1-phenylethyl acetate High enantiomeric excess
通讯作者	Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China. ; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China. ; Hu, YF (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China.
中文摘要	A novel microbial esterase BSE01281 identified from the Indian Ocean was cloned, expressed, and functionally characterized. Esterase BSE01281 could enanoselectively resolve (+/-)-1-phenylethanol and (+/-)-1-phenylethyl acetate through two types of enzymatic reactions. After the optimization of enzymatic reactions, BSE01281 could efficiently generate (R)-1-phenylethyl acetate with high enantiomeric excess (> 99 %) and high conversion (42 %) after 96 h trans-esterification reactions. Additionally, BSE01281 could also produce (R)-1-phenylethanol (e.e. > 99 %) and (S)-1-phenylethyl acetate (e.e. > 95 %) at a conversion of 49 % through direct hydrolysis of inexpensive racemic 1-phenylethyl acetate for 8 h. Optically pure (R)-1-phenylethanol generated from direct enzymatic hydrolysis of racemic 1-phenylethyl acetate by BSE01281 is not easily prepared by dehydrogenases, which generally follow the "Prelog's rule" and give (S)-1-phenylethanol instead.
学科主题	Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/15498]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Liang, Jiayuan,Zhang, Yun,Sun, Aijun,et al. Enantioselective Resolution of (+/-)-1-Phenylethanol and (+/-)-1-Phenylethyl Acetate by a Novel Esterase from Bacillus sp SCSIO 15121[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2016,178(3):558-575.
APA	Liang, Jiayuan,Zhang, Yun,Sun, Aijun,Deng, Dun,&Hu, Yunfeng.(2016).Enantioselective Resolution of (+/-)-1-Phenylethanol and (+/-)-1-Phenylethyl Acetate by a Novel Esterase from Bacillus sp SCSIO 15121.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,178(3),558-575.
MLA	Liang, Jiayuan,et al."Enantioselective Resolution of (+/-)-1-Phenylethanol and (+/-)-1-Phenylethyl Acetate by a Novel Esterase from Bacillus sp SCSIO 15121".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 178.3(2016):558-575.