Functional Characterization of a Novel Marine Microbial GDSL Lipase and Its Utilization in the Resolution of (+/-)-1-Phenylethanol

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Functional Characterization of a Novel Marine Microbial GDSL Lipase and Its Utilization in the Resolution of (+/-)-1-Phenylethanol
	Deng, Dun; Zhang, Yun; Sun, Aijun; Liang, Jiayuan; Hu, Yunfeng
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2016
卷号	179 期号:1 页码:75-93
关键词	Novel GDSL lipase Kinetic resolution (R)-1-phenylethanol Opposite optical selectivity
通讯作者	Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China. ; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China. ; Hu, YF (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China.
中文摘要	A novel GDSL lipase (MT6) was cloned from the genome of Marinactinospora thermotolerans SCSIO 00652 identified from the South China Sea. MT6 showed its maximum identity of 59 % with a putative lipase from Nocardiopsis dassonville. MT6 was heterologously expressed in E. coli BL21(DE3) and further functionally characterized. MT6 could efficiently resolve racemic 1-phenylethanol and generate (R)-1-phenylethanol with high enantiomeric excess (99 %) and conversion rate (54 %) through transesterification reactions after process optimization. Our report was the first one report about the utilization of one GDSL lipase in the preparation of chiral chemicals by transesterification reactions, and the optical selectivity of MT6 was interestingly opposite to those of other common lipases. GDSL lipases represented by MT6 possess great potential for the generation of valuable chiral chemicals in industry.
学科主题	Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/15465]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Deng, Dun,Zhang, Yun,Sun, Aijun,et al. Functional Characterization of a Novel Marine Microbial GDSL Lipase and Its Utilization in the Resolution of (+/-)-1-Phenylethanol[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2016,179(1):75-93.
APA	Deng, Dun,Zhang, Yun,Sun, Aijun,Liang, Jiayuan,&Hu, Yunfeng.(2016).Functional Characterization of a Novel Marine Microbial GDSL Lipase and Its Utilization in the Resolution of (+/-)-1-Phenylethanol.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,179(1),75-93.
MLA	Deng, Dun,et al."Functional Characterization of a Novel Marine Microbial GDSL Lipase and Its Utilization in the Resolution of (+/-)-1-Phenylethanol".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 179.1(2016):75-93.