A novel roseobacter phage possesses features of podoviruses, siphoviruses, prophages and gene transfer agents

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	A novel roseobacter phage possesses features of podoviruses, siphoviruses, prophages and gene transfer agents
	Zhan, Yuanchao; Huang, Sijun; Voget, Sonja; Simon, Meinhard; Chen, Feng
刊名	SCIENTIFIC REPORTS
	2016
卷号	6 页码:30372
通讯作者	Chen, F (reprint author), Univ Maryland, Inst Marine & Environm Technol, Ctr Environm Sci, College Pk, MD 20742 USA.
中文摘要	Bacteria in the Roseobacter lineage have been studied extensively due to their significant biogeochemical roles in the marine ecosystem. However, our knowledge on bacteriophage which infects the Roseobacter clade is still very limited. Here, we report a new bacteriophage, phage DSS3 Phi 8, which infects marine roseobacter Ruegeria pomeroyi DSS-3. DSS3 Phi 8 is a lytic siphovirus. Genomic analysis showed that DSS3 Phi 8 is most closely related to a group of siphoviruses, CbK-like phages, which infect freshwater bacterium Caulobacter crescentus. DSS3 Phi 8 contains a smaller capsid and has a reduced genome size (146 kb) compared to the CbK-like phages (205-279 kb). DSS3 Phi 8 contains the DNA polymerase gene which is closely related to T7-like podoviruses. DSS3 Phi 8 also contains the integrase and repressor genes, indicating its potential to involve in lysogenic cycle. In addition, four GTA (gene transfer agent) genes were identified in the DSS3 Phi 8 genome. Genomic analysis suggests that DSS3 Phi 8 is a highly mosaic phage that inherits the genetic features from siphoviruses, podoviruses, prophages and GTAs. This is the first report of CbK-like phages infecting marine bacteria. We believe phage isolation is still a powerful tool that can lead to discovery of new phages and help interpret the overwhelming unknown sequences in the viral metagenomics.
学科主题	Science & Technology - Other Topics
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/15380]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Zhan, Yuanchao,Huang, Sijun,Voget, Sonja,et al. A novel roseobacter phage possesses features of podoviruses, siphoviruses, prophages and gene transfer agents[J]. SCIENTIFIC REPORTS,2016,6:30372.
APA	Zhan, Yuanchao,Huang, Sijun,Voget, Sonja,Simon, Meinhard,&Chen, Feng.(2016).A novel roseobacter phage possesses features of podoviruses, siphoviruses, prophages and gene transfer agents.SCIENTIFIC REPORTS,6,30372.
MLA	Zhan, Yuanchao,et al."A novel roseobacter phage possesses features of podoviruses, siphoviruses, prophages and gene transfer agents".SCIENTIFIC REPORTS 6(2016):30372.