Genomic structure and expression pattern of MHC II alpha and II beta genes reveal an unusual immune trait in lined seahorse Hippocampus erectus

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	Genomic structure and expression pattern of MHC II alpha and II beta genes reveal an unusual immune trait in lined seahorse Hippocampus erectus
	Luo, Wei; Wang, Xin; Qu, Hongyue; Qin, Geng; Zhang, Huixian; Lin, Qiang
刊名	FISH & SHELLFISH IMMUNOLOGY
	2016
卷号	58 页码:521-529
关键词	Major histocompatibility complex II Polymorphism Genomic structure Selective pressure Hippocampus erectus
通讯作者	Lin, Q (reprint author), 164 Xingang West Rd, Guangzhou 510301, Guangdong, Peoples R China.
英文摘要	The major histocompatibility complex (MHC) genes are crucial in the adaptive immune system, and the gene duplication of MHC in animals can generally result in immune flexibility. In this study, we found that the lined seahorse (Hippocampus erectus) has only one gene copy number (GCN) of MHC II alpha and II beta, which is different from that in other teleosts. Together with the lack of spleen and gut-associated lymphatic tissue (GALT), the seahorse may be referred to as having a partial but natural "immunodeficiency". Highly variable amino acid residues were found in the II alpha and 1113 domains, especially in the alpha 1 and beta 1 domains with 9.62% and 8.43% allelic variation, respectively. Site models revealed seven and ten positively selected positions in the alpha 1 and beta 1 domains, respectively. Real-time PCR experiments showed high expression levels of the MHC II genes in intestine (In), gill (Gi) and trunk kidney (TK) and medium in muscle (Mu) and brood pouch (BP), and the expression levels were significantly up-regulated after bacterial infection. Specially, relative higher expression level of both MHC II alpha and II beta was found in Mu and BP when compared with other fish species, in which MHC II is expressed negligibly in Mu. These results indicate that apart from TIC, Gi and In, MU and BP play an important role in the immune response against pathogens in the seahorse. In conclusion, high allelic variation and strong positive selection in PBR and relative higher expression in MU and BP are speculated to partly compensate for the immunodeficiency. (C) 2016 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/15265]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Luo, Wei,Wang, Xin,Qu, Hongyue,et al. Genomic structure and expression pattern of MHC II alpha and II beta genes reveal an unusual immune trait in lined seahorse Hippocampus erectus[J]. FISH & SHELLFISH IMMUNOLOGY,2016,58:521-529.
APA	Luo, Wei,Wang, Xin,Qu, Hongyue,Qin, Geng,Zhang, Huixian,&Lin, Qiang.(2016).Genomic structure and expression pattern of MHC II alpha and II beta genes reveal an unusual immune trait in lined seahorse Hippocampus erectus.FISH & SHELLFISH IMMUNOLOGY,58,521-529.
MLA	Luo, Wei,et al."Genomic structure and expression pattern of MHC II alpha and II beta genes reveal an unusual immune trait in lined seahorse Hippocampus erectus".FISH & SHELLFISH IMMUNOLOGY 58(2016):521-529.